ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MATLIDNRYG KARVRVLRVF KGPNEYHKVF DFDCRVLLRG AEFSETYLTG DNSKVVATDT 

        70         80         90        100        110        120 
MKNTVYVIAQ KEEFKSLEEY GILLGKHFLA TYSWVNGVEV VMRENQWRRI KTSNGKEQAH 

       130        140        150        160        170        180 
SFQRDREIHS VTVTSSRDKS PVVVSGIDDL LIMKTTQSGF EGFHRDKYTS LKETKDRVFA 

       190        200        210        220        230        240 
TVVTANWTYN TLSVDYSKVF EQFKLSVFDI FAQTYSRSVQ ETLFLIAKDV ISKVPQVEQV 

       250        260        270        280 
HLSLPNKHAF GFDFSRLNIE NNQTVFQPVE EPSGLIEGTI KRSHSRL 

References and documentation are available.

Number of amino acids: 287

Molecular weight: 33031.49

Theoretical pI: 8.95

Amino acid composition: 
Ala (A)  12	  4.2%
Arg (R)  18	  6.3%
Asn (N)  13	  4.5%
Asp (D)  15	  5.2%
Cys (C)   1	  0.3%
Gln (Q)  12	  4.2%
Glu (E)  20	  7.0%
Gly (G)  14	  4.9%
His (H)   8	  2.8%
Ile (I)  13	  4.5%
Leu (L)  21	  7.3%
Lys (K)  21	  7.3%
Met (M)   4	  1.4%
Phe (F)  19	  6.6%
Pro (P)   6	  2.1%
Ser (S)  24	  8.4%
Thr (T)  22	  7.7%
Trp (W)   3	  1.0%
Tyr (Y)  10	  3.5%
Val (V)  31	 10.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 39

Atomic composition:

Carbon      C	      1484
Hydrogen    H	      2318
Nitrogen    N	       406
Oxygen      O	       439
Sulfur      S	         5

Formula: C1484H2318N406O439S5
Total number of atoms: 4652

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    31400
Abs 0.1% (=1 g/l)   0.951, assuming all pairs of Cys residues form cystines


Ext. coefficient    31400
Abs 0.1% (=1 g/l)   0.951, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.44
This classifies the protein as stable.



Aliphatic index: 81.71

Grand average of hydropathicity (GRAVY): -0.385