ProtParam
User-provided sequence:
10 20 30 40 50 60
MATLKVSDSV PAPSDDAEQL RTAFEGWGTN EDLIISILAH RSAEQRKVIR QAYHETYGED
70 80 90 100 110 120
LLKTLDKELS NDFERAILLW TLEPGERDAL LANEATKRWT SSNQVLMEVA CTRTSTQLLH
130 140 150 160 170 180
ARQAYHARYK KSLEEDVAHH TTGDFRKLLV SLVTSYRYEG DEVNMTLAKQ EAKLVHEKIK
190 200 210 220 230 240
DKHYNDEDVI RILSTRSKAQ INATFNRYQD DHGEEILKSL EEGDDDDKFL ALLRSTIQCL
250 260 270 280 290 300
TRPELYFVDV LRSAINKTGT DEGALTRIVT TRAEIDLKVI GEEYQRRNSI PLEKAITKDT
310
RGDYEKMLVA LLGEDDA
References and
documentation are available.
Number of amino acids: 317
Molecular weight: 36203.64
Theoretical pI: 5.21
Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 44
Atomic composition:
Carbon C 1582
Hydrogen H 2541
Nitrogen N 449
Oxygen O 510
Sulfur S 6
Formula: C
1582H
2541N
449O
510S
6
Total number of atoms: 5088
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 33015
Abs 0.1% (=1 g/l) 0.912, assuming all pairs of Cys residues form cystines
Ext. coefficient 32890
Abs 0.1% (=1 g/l) 0.908, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.10
This classifies the protein as stable.
Aliphatic index: 89.59
Grand average of hydropathicity (GRAVY): -0.603