ProtParam
User-provided sequence:
10 20 30 40 50 60
MATLVRRAAS RFVGSYWKNR SFVYSLLDQS ASGRRRHLTP SVDRNVPFTT STKKRASPTD
70 80 90 100 110 120
PLLRIIEEEI GYAEKADDYH RVEETPSGFP FEMEDKPGGK IVTLTREYEG ETVKVEVHMT
130 140 150 160 170 180
NLVTGDKEDD EDDEEEAENE EDEDEDKPLK PKQSNVPLLV TLSKKTGPSL EFRCTAFPDR
190 200 210 220 230 240
IAIKDMWVTF PDDPSKDELA YEGPSFRVLD EKLRKAFHRY IEIRGIKPSM INFLHEYMIN
250
KDSREHLLWL KSLKNFVKY
References and
documentation are available.
Number of amino acids: 259
Molecular weight: 30068.89
Theoretical pI: 5.36
Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 41
Atomic composition:
Carbon C 1336
Hydrogen H 2089
Nitrogen N 363
Oxygen O 413
Sulfur S 7
Formula: C
1336H
2089N
363O
413S
7
Total number of atoms: 4208
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 29910
Abs 0.1% (=1 g/l) 0.995, assuming all pairs of Cys residues form cystines
Ext. coefficient 29910
Abs 0.1% (=1 g/l) 0.995, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.81
This classifies the protein as unstable.
Aliphatic index: 70.73
Grand average of hydropathicity (GRAVY): -0.791