ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MATNIGMMDS AYFVGRNEIL SWINDRLHLN LSRIEEAASG AVQCQMLDMT FPGVVPMHKV 

        70         80         90        100        110        120 
NFEAKNEYEM IQNYKVMQEV FTKLKITKPL EVNRLVKGRP LDNLEFLQWL KRFCDSINGG 

       130        140        150        160        170        180 
IMNENYNPVE RRSRGGREKS VKGSSKISKS LQTNNMHHPP VATSNKPAGP KQAKSHGIGG 

       190        200        210        220        230        240 
GSNSSAEVQA LSKEVEDLKV SVDLLEKERD FYFSKLRDIE ILCQTPELDD LPIVVAVKKI 

       250        260        270        280        290 
LYATDANESV LEEAQECLNQ SLGLEGYEEE GKEEEEEEEE EEEEAAAAAE TQT 

References and documentation are available.

Number of amino acids: 293

Molecular weight: 32931.09

Theoretical pI: 4.84

Amino acid composition: 
Ala (A)  20	  6.8%
Arg (R)  12	  4.1%
Asn (N)  19	  6.5%
Asp (D)  12	  4.1%
Cys (C)   4	  1.4%
Gln (Q)  12	  4.1%
Glu (E)  40	 13.7%
Gly (G)  18	  6.1%
His (H)   5	  1.7%
Ile (I)  14	  4.8%
Leu (L)  26	  8.9%
Lys (K)  22	  7.5%
Met (M)  10	  3.4%
Phe (F)   8	  2.7%
Pro (P)  11	  3.8%
Ser (S)  21	  7.2%
Thr (T)  10	  3.4%
Trp (W)   2	  0.7%
Tyr (Y)   7	  2.4%
Val (V)  20	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      1431
Hydrogen    H	      2286
Nitrogen    N	       394
Oxygen      O	       467
Sulfur      S	        14

Formula: C1431H2286N394O467S14
Total number of atoms: 4592

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21680
Abs 0.1% (=1 g/l)   0.658, assuming all pairs of Cys residues form cystines


Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.651, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 57.22
This classifies the protein as unstable.



Aliphatic index: 79.86

Grand average of hydropathicity (GRAVY): -0.589