ProtParam
User-provided sequence:
10 20 30 40 50 60
MATNIGMMDS AYFVGRNEIL TWINDRLHLN LSRVEEAASG AVQCQMLDMT FPGVVPMHKV
70 80 90 100 110 120
NFDAKNEYDM IQNYKVLQDV FNKLKITKPL EINRLVKGRP LDNLEFLQWL KRFCDSINGG
130 140 150 160 170 180
IMNENYNPVE RRSRNGKERS VKGSNKIPKS LQTNNNHPPP NSSSVGLSKA SGPKSAKAAE
190 200 210 220 230 240
VQALSKELVD LKISTDLLEK ERDFYFSKLR DVEILCQTPE LDDLPIVVAV KKILYATDAN
250 260 270
ESALEDAQEY LNQSLGVEDD EAEGNGEQLE EEKTQA
References and
documentation are available.
Number of amino acids: 276
Molecular weight: 31080.18
Theoretical pI: 5.08
Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 34
Atomic composition:
Carbon C 1359
Hydrogen H 2179
Nitrogen N 377
Oxygen O 433
Sulfur S 11
Formula: C
1359H
2179N
377O
433S
11
Total number of atoms: 4359
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 21555
Abs 0.1% (=1 g/l) 0.694, assuming all pairs of Cys residues form cystines
Ext. coefficient 21430
Abs 0.1% (=1 g/l) 0.690, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.75
This classifies the protein as unstable.
Aliphatic index: 85.83
Grand average of hydropathicity (GRAVY): -0.557