ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MATPHLYHRY NSKSSNKNIN SSGNSTEVDR FIPKSVSRNA YKSIPMLNGF DISYSELCEK 

        70         80         90        100        110        120 
SPSPERLSSP EFFNELRNTG HYESISTTNE FSMSSISSSS ESQVTRSGSA RASRNDYSKL 

       130        140        150        160        170        180 
TKEQKDHRKN IAHSLGFQLP DRVFTFETTS AEILEKNKAI KNCFGPGSCA EIRSTFDFST 

       190        200        210        220        230        240 
LSPDVARYYI ANSNARSASP QRQIQRPAKR VKSHIPYRVL DAPCLRNDFY SNLISWSRTT 

       250        260        270        280        290        300 
NNVLVGLGCS VYIWSEKEGA VSILDHQYLS EKRDLVTCVS FCPYNTYFIV GTKFGRILLY 

       310        320        330        340        350        360 
DQKEFFHSSN TNEKEPVFVF QTESFKGICC LEWFKPGEIC KFYVGEENGN VSLFEIKSLH 

       370        380        390        400        410        420 
FPIKNWSKRQ KLEDENLIGL KLHSTYQAQA QQVCGISLNE HANLLAVGGN DNSCSLWDIS 

       430        440        450        460        470        480 
DLDKPIKKFV LPHKAAVKAI AFCPWSKSLL ATGGGSKDRC IKFWHTSTGT LLDEICTSGQ 

       490        500        510        520        530        540 
VTSLIWSLRH KQIVATFGFG DTKNPVLITL YSYPKLSKLL EVRSPNPLRV LSAVISPSSM 

       550        560 
AICVATNDET IRFMNCGTIR RK 

References and documentation are available.

Number of amino acids: 562

Molecular weight: 63275.83

Theoretical pI: 9.05

Amino acid composition: 
Ala (A)  28	  5.0%
Arg (R)  29	  5.2%
Asn (N)  35	  6.2%
Asp (D)  21	  3.7%
Cys (C)  17	  3.0%
Gln (Q)  16	  2.8%
Glu (E)  32	  5.7%
Gly (G)  28	  5.0%
His (H)  14	  2.5%
Ile (I)  36	  6.4%
Leu (L)  46	  8.2%
Lys (K)  40	  7.1%
Met (M)   5	  0.9%
Phe (F)  30	  5.3%
Pro (P)  25	  4.4%
Ser (S)  72	 12.8%
Thr (T)  32	  5.7%
Trp (W)   8	  1.4%
Tyr (Y)  19	  3.4%
Val (V)  29	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 69

Atomic composition:

Carbon      C	      2813
Hydrogen    H	      4392
Nitrogen    N	       776
Oxygen      O	       843
Sulfur      S	        22

Formula: C2813H4392N776O843S22
Total number of atoms: 8846

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    73310
Abs 0.1% (=1 g/l)   1.159, assuming all pairs of Cys residues form cystines


Ext. coefficient    72310
Abs 0.1% (=1 g/l)   1.143, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.43
This classifies the protein as unstable.



Aliphatic index: 76.85

Grand average of hydropathicity (GRAVY): -0.380