ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MATPMVEDTS SFEEDQLASM STEDITRATR LLDNEIRILK EDAQRTNLEC DSYKEKIKEN 

        70         80         90        100        110        120 
QEKIKLNKQL PYLVGNIVEI LEMNPEDDAE EDGANIDLDS QRKGKCVVLK TSTRQTIFLP 

       130        140        150        160        170        180 
VVGLVDPDSL KPGDLVGVNK DSYLILDTLP SEYDSRVKAM EVDEKPTEDY NDIGGLEKQI 

       190        200        210        220        230        240 
QELVEAIVLP MTHKERFEKL GVRPPKGVLL YGPPGTGKTL MARACAAQTN ATFLKLAGPQ 

       250        260        270        280        290        300 
LVQMFIGDGA KLVRDAFQLA KEKAPCIIFI DEIDAIGTKR FDSEVSGDRE VQRTMLELLN 

       310        320        330        340        350        360 
QLDGFSSDER IKVIAATNRA DILDPALMRS GRLDRKIEFP HPTEEARARI LQIHSRKMNV 

       370        380        390        400        410        420 
HPDVNFEELA RSTDDFNGAQ LKAVCVEAGM LALRRDATEV NHEDFNEGII QVQAKKKASL 


NYYA 

References and documentation are available.

Number of amino acids: 424

Molecular weight: 47480.06

Theoretical pI: 4.91

Amino acid composition: 
Ala (A)  34	  8.0%
Arg (R)  25	  5.9%
Asn (N)  18	  4.2%
Asp (D)  36	  8.5%
Cys (C)   5	  1.2%
Gln (Q)  18	  4.2%
Glu (E)  39	  9.2%
Gly (G)  23	  5.4%
His (H)   5	  1.2%
Ile (I)  27	  6.4%
Leu (L)  43	 10.1%
Lys (K)  31	  7.3%
Met (M)  12	  2.8%
Phe (F)  13	  3.1%
Pro (P)  19	  4.5%
Ser (S)  19	  4.5%
Thr (T)  22	  5.2%
Trp (W)   0	  0.0%
Tyr (Y)   8	  1.9%
Val (V)  27	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 75
Total number of positively charged residues (Arg + Lys): 56

Atomic composition:

Carbon      C	      2074
Hydrogen    H	      3370
Nitrogen    N	       576
Oxygen      O	       660
Sulfur      S	        17

Formula: C2074H3370N576O660S17
Total number of atoms: 6697

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    12170
Abs 0.1% (=1 g/l)   0.256, assuming all pairs of Cys residues form cystines


Ext. coefficient    11920
Abs 0.1% (=1 g/l)   0.251, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.96
This classifies the protein as unstable.



Aliphatic index: 90.87

Grand average of hydropathicity (GRAVY): -0.442