ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MATPTESELA SPIPQTNPGS YEELHRKARD VFPTCFEGAK LMVNKGLSSH FQVSHTLSLS 

        70         80         90        100        110        120 
AMNTGYRFGA TYVGTNQVGP AEAYPILLGD TDVNGNTTAT ILHQLGIYRT KLQGQIQQGK 

       130        140        150        160        170        180 
LAGAQATIER KGRLSTLGLT LANIDLVNEA GILVGQFLRR LTPRLDVGTE MVYQYGKNIP 

       190        200        210        220        230        240 
GGQISVLSYA ARYTANHFIA AATLGASGVH LTYYHKQNEN LAFGVEFECN ANVGEAVTTL 

       250        260        270        280        290        300 
AYQTELPEEG VTMRASFDTN WTVGGVFEKR LSQQLPFTLA LSGTLNHVKA AGKFGIGLII 


G 

References and documentation are available.

Number of amino acids: 301

Molecular weight: 32385.75

Theoretical pI: 7.10

Amino acid composition: 
Ala (A)  29	  9.6%
Arg (R)  12	  4.0%
Asn (N)  16	  5.3%
Asp (D)   6	  2.0%
Cys (C)   2	  0.7%
Gln (Q)  16	  5.3%
Glu (E)  17	  5.6%
Gly (G)  33	 11.0%
His (H)   8	  2.7%
Ile (I)  14	  4.7%
Leu (L)  33	 11.0%
Lys (K)  11	  3.7%
Met (M)   5	  1.7%
Phe (F)  12	  4.0%
Pro (P)  11	  3.7%
Ser (S)  15	  5.0%
Thr (T)  29	  9.6%
Trp (W)   1	  0.3%
Tyr (Y)  12	  4.0%
Val (V)  19	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon      C	      1443
Hydrogen    H	      2275
Nitrogen    N	       397
Oxygen      O	       436
Sulfur      S	         7

Formula: C1443H2275N397O436S7
Total number of atoms: 4558

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    23505
Abs 0.1% (=1 g/l)   0.726, assuming all pairs of Cys residues form cystines


Ext. coefficient    23380
Abs 0.1% (=1 g/l)   0.722, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 23.90
This classifies the protein as stable.



Aliphatic index: 88.84

Grand average of hydropathicity (GRAVY): -0.087