ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MATRFDTGES IGSGKTSISR DEFAEDSLKI VSLPPVENDD SVEEMISPKK QVKSSPFTSV 

        70         80         90        100        110        120 
FEKDGEIIHS DSIDISFSET TLEETVAMNV ECPGVVVVSV PENGSTILEE NKVGEEIKES 

       130        140        150        160        170        180 
SEISTTEGDR LGDDVLKIKD VVETDGFVVS ISDMKESLNS EKKGQDSDGQ RIVEAEKIIL 

       190        200        210        220        230        240 
FAEIGDGSTF LEENKELQQI KEVSEISTES DQFGDDVYKI EDDCVGSISD MKEILNSQKK 

       250        260        270        280        290        300 
GQDSDVQILW ETEKKNDGDT LARASDIHKI EKNGNGSKDQ REKVERVRVK DNAFVGRSVN 

       310        320        330        340        350        360 
IDLVDDTALF DVVPFYKKGK DHSKRPVTAH TDKDAPRKHK KVGVEKPIDK GNASSIVERN 

       370        380        390        400        410        420 
ASTKVSDFRN SGEMNGKQLR IMYSRNQMES MRLLPELVTE YEGLKNHKSI LEDYVVKEEK 

       430        440        450        460        470        480 
TEDNDDYNSI LRPAFAVDGE PDFDSGPPED GIEYLRRVRH IWLSCFHHLR WEAKRIPNVK 

       490        500        510        520        530        540 
VAKVSGSKYR EKEQSVYMPQ IPRAPATGEG MGGLVAFRLL THSSGIPFFQ LAYLSVFLLT 

       550        560        570        580        590        600 
DKDTRNGLSD TGIKAEKADM FGEKESGLES SDCKWVVALC ASVDTPPDAD TSACLRALVR 

       610        620        630        640 
KCASLRALEV GVLEMNKLSL WQICSSPSQA DILAKWNERR SVFTTV 

References and documentation are available.

Number of amino acids: 646

Molecular weight: 71910.61

Theoretical pI: 4.90

Amino acid composition: 
Ala (A)  32	  5.0%
Arg (R)  31	  4.8%
Asn (N)  24	  3.7%
Asp (D)  56	  8.7%
Cys (C)   8	  1.2%
Gln (Q)  17	  2.6%
Glu (E)  64	  9.9%
Gly (G)  41	  6.3%
His (H)  10	  1.5%
Ile (I)  39	  6.0%
Leu (L)  43	  6.7%
Lys (K)  56	  8.7%
Met (M)  13	  2.0%
Phe (F)  22	  3.4%
Pro (P)  23	  3.6%
Ser (S)  66	 10.2%
Thr (T)  30	  4.6%
Trp (W)   6	  0.9%
Tyr (Y)  10	  1.5%
Val (V)  55	  8.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 120
Total number of positively charged residues (Arg + Lys): 87

Atomic composition:

Carbon      C	      3128
Hydrogen    H	      5010
Nitrogen    N	       862
Oxygen      O	      1034
Sulfur      S	        21

Formula: C3128H5010N862O1034S21
Total number of atoms: 10055

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48400
Abs 0.1% (=1 g/l)   0.673, assuming all pairs of Cys residues form cystines


Ext. coefficient    47900
Abs 0.1% (=1 g/l)   0.666, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.26
This classifies the protein as unstable.



Aliphatic index: 79.15

Grand average of hydropathicity (GRAVY): -0.565