ProtParam
User-provided sequence:
10 20 30 40 50 60
MATRVVATAT VRAVKGRKLI PTRAALTLTP AAVLRIKTLL QDKPDMVGLK VGVRQRGCNG
70 80 90 100 110 120
LSYTLDYASQ KDKLDEEVVQ DGVKVFIDKK AQLSLLGTEM DFVESKLSSE FVFNNPNIKG
130
TCGCGESFSM
References and
documentation are available.
Number of amino acids: 130
Molecular weight: 14140.49
Theoretical pI: 9.24
Total number of negatively charged residues (Asp + Glu): 14
Total number of positively charged residues (Arg + Lys): 19
Atomic composition:
Carbon C 621
Hydrogen H 1032
Nitrogen N 172
Oxygen O 188
Sulfur S 7
Formula: C
621H
1032N
172O
188S
7
Total number of atoms: 2020
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 3105
Abs 0.1% (=1 g/l) 0.220, assuming all pairs of Cys residues form cystines
Ext. coefficient 2980
Abs 0.1% (=1 g/l) 0.211, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 20.62
This classifies the protein as stable.
Aliphatic index: 92.92
Grand average of hydropathicity (GRAVY): -0.062