ProtParam
User-provided sequence:
10 20 30 40 50 60
MATTSFSLPN HKFGIKLMLF LVLNLLSLQT SVFAHSSNSK FTKISRHPNS DSSSRTKPST
70 80 90 100 110 120
SSNKGFLSSV QLSLDHALFA RSLAFNLTLS HRTSQTLMLD PVNDCLELLD DTLDMLYRIV
130 140 150 160 170 180
VIKRKDHVND DVHTWLSAAL TNQETCKQSL SEKSSFNKEG IAIDSFARNL TGLLTNSLDM
190 200 210 220 230 240
FVSDKQKSSS SSNLTGGRKL LSDHDFPTWV SSSDRKLLEA SVEELRPHAV VAADGSGTHM
250 260 270 280 290 300
SVAEALASLE KGSGRSVIHL TAGTYKENLN IPSKQKNVML VGDGKGKTVI VGSRSNRGGW
310 320 330 340 350 360
NTYQSATVAA MGDGFIARDI TFVNSAGPNS EQAVALRVGS DRSVVYRCSI DGYQDSLYTL
370 380 390 400 410 420
SKRQFYRETD ITGTVDFIFG NSAVVFQSCN LVSRKGSSDQ NYVTAQGRSD PNQNTGISIH
430 440 450 460 470 480
NCRITGSTKT YLGRPWKQYS RTVVMQSFID GSIHPSGWSP WSSNFALKTL YYGEFGNSGP
490 500 510 520
GSSVSGRVSW AGYHPALTLT EAQGFTVSGF IDGNSWLPST GVVFDSGLL
References and
documentation are available.
Number of amino acids: 529
Molecular weight: 57513.46
Theoretical pI: 9.00
Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 51
Atomic composition:
Carbon C 2526
Hydrogen H 3971
Nitrogen N 713
Oxygen O 796
Sulfur S 14
Formula: C
2526H
3971N
713O
796S
14
Total number of atoms: 8020
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 63620
Abs 0.1% (=1 g/l) 1.106, assuming all pairs of Cys residues form cystines
Ext. coefficient 63370
Abs 0.1% (=1 g/l) 1.102, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.38
This classifies the protein as stable.
Aliphatic index: 79.04
Grand average of hydropathicity (GRAVY): -0.262