ProtParam
User-provided sequence:
10 20 30 40 50 60
MATTSTTTKL SVLCCSFISS PLVDSPPSLA FFSPIPRFLT VRIATSFRSS SRFPATKIRK
70 80 90 100 110 120
SSLAAVMFPE NSVLSDVCAF GVTSIVAFSC LGFWGEIGKR GIFDQKLIRK LVHINIGLVF
130 140 150 160 170 180
MLCWPLFSSG IQGALFASLV PGLNIVRMLL LGLGVYHDEG TIKSMSRHGD RRELLKGPLY
190 200 210 220 230 240
YVLSITSACI YYWKSSPIAI AVICNLCAGD GMADIVGRRF GTEKLPYNKN KSFAGSIGMA
250 260 270 280 290 300
TAGFLASVAY MYYFASFGYI EDSGGMILRF LVISIASALV ESLPISTDID DNLTISLTSA
LAGFLLF
References and
documentation are available.
Number of amino acids: 307
Molecular weight: 33218.05
Theoretical pI: 9.11
Total number of negatively charged residues (Asp + Glu): 18
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 1522
Hydrogen H 2393
Nitrogen N 379
Oxygen O 417
Sulfur S 17
Formula: C
1522H
2393N
379O
417S
17
Total number of atoms: 4728
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 31900
Abs 0.1% (=1 g/l) 0.960, assuming all pairs of Cys residues form cystines
Ext. coefficient 31400
Abs 0.1% (=1 g/l) 0.945, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.92
This classifies the protein as unstable.
Aliphatic index: 106.12
Grand average of hydropathicity (GRAVY): 0.586