ProtParam
User-provided sequence:
10 20 30 40 50 60
MATVHQKNMS TLKQRKEDFV TGLNGGSITE INAVTSIALV TYISWNLLKN SNLMPPGISS
70 80 90 100 110 120
VQYIIDFALN WVALLLSITI YASEPYLLNT LILLPCLLAF IYGKFTSSSK PSNPIYNKKK
130 140 150 160 170 180
MITQRFQLEK KPYITAYRGG MLILTAIAIL AVDFPIFPRR FAKVETWGTS LMDLGVGSFV
190 200 210 220 230 240
FSNGIVSSRA LLKNLSLKSK PSFLKNAFNA LKSGGTLLFL GLLRLFFVKN LEYQEHVTEY
250 260 270 280 290 300
GVHWNFFITL SLLPLVLTFI DPVTRMVPRC SIAIFISCIY EWLLLKDDRT LNFLILADRN
310 320 330 340 350 360
CFFSANREGI FSFLGYCSIF LWGQNTGFYL LGNKPTLNNL YKPSTQDVVA ASKKSSTWDY
370 380 390 400 410 420
WTSVTPLSGL CIWSTIFLVI SQLVFQYHPY SVSRRFANLP YTLWVITYNL LFLTGYCLTD
430 440 450 460 470 480
KIFGNSSEYY KVAECLESIN SNGLFLFLLA NVSTGLVNMS MVTIDSSPLK SFLVLLAYCS
490
FIAVISVFLY RKRIFIKL
References and
documentation are available.
Number of amino acids: 498
Molecular weight: 56376.38
Theoretical pI: 9.45
Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 44
Atomic composition:
Carbon C 2640
Hydrogen H 4086
Nitrogen N 632
Oxygen O 695
Sulfur S 18
Formula: C
2640H
4086N
632O
695S
18
Total number of atoms: 8071
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 91260
Abs 0.1% (=1 g/l) 1.619, assuming all pairs of Cys residues form cystines
Ext. coefficient 90760
Abs 0.1% (=1 g/l) 1.610, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 24.82
This classifies the protein as stable.
Aliphatic index: 112.35
Grand average of hydropathicity (GRAVY): 0.431