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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MATVHQKNMS TLKQRKEDFV TGLNGGSITE INAVTSIALV TYISWNLLKN SNLMPPGISS

70 80 90 100 110 120
VQYIIDFALN WVALLLSITI YASEPYLLNT LILLPCLLAF IYGKFTSSSK PSNPIYNKKK

130 140 150 160 170 180
MITQRFQLEK KPYITAYRGG MLILTAIAIL AVDFPIFPRR FAKVETWGTS LMDLGVGSFV

190 200 210 220 230 240
FSNGIVSSRA LLKNLSLKSK PSFLKNAFNA LKSGGTLLFL GLLRLFFVKN LEYQEHVTEY

250 260 270 280 290 300
GVHWNFFITL SLLPLVLTFI DPVTRMVPRC SIAIFISCIY EWLLLKDDRT LNFLILADRN

310 320 330 340 350 360
CFFSANREGI FSFLGYCSIF LWGQNTGFYL LGNKPTLNNL YKPSTQDVVA ASKKSSTWDY

370 380 390 400 410 420
WTSVTPLSGL CIWSTIFLVI SQLVFQYHPY SVSRRFANLP YTLWVITYNL LFLTGYCLTD

430 440 450 460 470 480
KIFGNSSEYY KVAECLESIN SNGLFLFLLA NVSTGLVNMS MVTIDSSPLK SFLVLLAYCS

490
FIAVISVFLY RKRIFIKL


References and documentation are available.

Number of amino acids: 498 Molecular weight: 56376.38 Theoretical pI: 9.45
Amino acid composition: 
Ala (A) 25 5.0% Arg (R) 16 3.2% Asn (N) 30 6.0% Asp (D) 12 2.4% Cys (C) 9 1.8% Gln (Q) 10 2.0% Glu (E) 13 2.6% Gly (G) 25 5.0% His (H) 4 0.8% Ile (I) 39 7.8% Leu (L) 75 15.1% Lys (K) 28 5.6% Met (M) 9 1.8% Phe (F) 37 7.4% Pro (P) 19 3.8% Ser (S) 49 9.8% Thr (T) 33 6.6% Trp (W) 10 2.0% Tyr (Y) 24 4.8% Val (V) 31 6.2% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 25 Total number of positively charged residues (Arg + Lys): 44 Atomic composition: Carbon C 2640 Hydrogen H 4086 Nitrogen N 632 Oxygen O 695 Sulfur S 18 Formula: C2640H4086N632O695S18 Total number of atoms: 8071 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 91260 Abs 0.1% (=1 g/l) 1.619, assuming all pairs of Cys residues form cystines Ext. coefficient 90760 Abs 0.1% (=1 g/l) 1.610, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 24.82 This classifies the protein as stable. Aliphatic index: 112.35 Grand average of hydropathicity (GRAVY): 0.431