ProtParam
User-provided sequence:
10 20 30 40 50 60
MATYQPYNEY SSVTGGGFEN SESRPGSGES ETNTRVNTLT PVTIKQILES KQDIQDGPFV
70 80 90 100 110 120
SHNQELHHVC FVGVVRNITD HTANIFLTIE DGTGQIEVRK WSEDANDLAA GNDDSSGKGY
130 140 150 160 170 180
GSQVAQQFEI GGYVKVFGAL KEFGGKKNIQ YAVIKPIDSF NEVLTHHLEV IKCHSIASGM
190 200 210 220 230 240
MKQPLESASN NNGQSLFVKD DNDTSSGSSP LQRILEFCKK QCEGKDANSF AVPIPLISQS
250 260 270
LNLDETTVRN CCTTLTDQGF IYPTFDDNNF FAL
References and
documentation are available.
Number of amino acids: 273
Molecular weight: 29936.14
Theoretical pI: 4.85
Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 21
Atomic composition:
Carbon C 1308
Hydrogen H 2025
Nitrogen N 357
Oxygen O 431
Sulfur S 9
Formula: C
1308H
2025N
357O
431S
9
Total number of atoms: 4130
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 16305
Abs 0.1% (=1 g/l) 0.545, assuming all pairs of Cys residues form cystines
Ext. coefficient 15930
Abs 0.1% (=1 g/l) 0.532, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.24
This classifies the protein as unstable.
Aliphatic index: 72.45
Grand average of hydropathicity (GRAVY): -0.447