ProtParam
User-provided sequence:
10 20 30 40 50 60
MAVEENNMPV VSQQPQAGED VISSLSKDSH LSAQSQKYSN DELKAGESGS EGSQSVPIEI
70 80 90 100 110 120
PKKPMSEYVT VSLLCLCVAF GGFMFGWDTG TISGFVVQTD FLRRFGMKHK DGTHYLSNVR
130 140 150 160 170 180
TGLIVAIFNI GCAFGGIILS KGGDMYGRKK GLSIVVSVYI VGIIIQIASI NKWYQYFIGR
190 200 210 220 230 240
IISGLGVGGI AVLCPMLISE IAPKHLRGTL VSCYQLMITA GIFLGYCTNY GTKSYSNSVQ
250 260 270 280 290 300
WRVPLGLCFA WSLFMIGALT LVPESPRYLC EVNKVEDAKR SIAKSNKVSP EDPAVQAELD
310 320 330 340 350 360
LIMAGIEAEK LAGNASWGEL FSTKTKVFQR LLMGVFVQMF QQLTGNNYFF YYGTVIFKSV
370 380 390 400 410 420
GLDDSFETSI VIGVVNFAST FFSLWTVENL GHRKCLLLGA ATMMACMVIY ASVGVTRLYP
430 440 450 460 470 480
HGKSQPSSKG AGNCMIVFTC FYIFCYATTW APVAWVITAE SFPLRVKSKC MALASASNWV
490 500 510 520 530 540
WGFLIAFFTP FITSAINFYY GYVFMGCLVA MFFYVFFFVP ETKGLSLEEI QELWEEGVLP
550 560 570
WKSEGWIPSS RRGNNYDLED LQHDDKPWYK AMLE
References and
documentation are available.
Number of amino acids: 574
Molecular weight: 63625.92
Theoretical pI: 7.00
Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 45
Atomic composition:
Carbon C 2920
Hydrogen H 4456
Nitrogen N 716
Oxygen O 807
Sulfur S 35
Formula: C
2920H
4456N
716O
807S
35
Total number of atoms: 8934
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 115125
Abs 0.1% (=1 g/l) 1.809, assuming all pairs of Cys residues form cystines
Ext. coefficient 114250
Abs 0.1% (=1 g/l) 1.796, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.04
This classifies the protein as unstable.
Aliphatic index: 91.34
Grand average of hydropathicity (GRAVY): 0.275