ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAVGKNKRLS KGKKGQKKRV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK STGLKSASDA 

        70         80         90        100        110        120 
LKGRVVEVCL ADLQGSEDHS FRKIKLRVDE VQGKNLLTNF HGMDFTTDKL RSMVRKWQTL 

       130        140        150        160        170        180 
IEANVTVKTS DDYVLRIFAI AFTRKQANQV KRHSYAQSSH IRAIRKVISE ILTKEVQGST 

       190        200        210        220        230        240 
LAQLTSKLIP EVINKEIENA TKDIFPLQNI HVRKVKLLKQ PKFDVGALMA LHGEGSGEEK 

       250 
GKKVTGFKDE VLETV 

References and documentation are available.

Number of amino acids: 255

Molecular weight: 28743.36

Theoretical pI: 10.00

Amino acid composition: 
Ala (A)  15	  5.9%
Arg (R)  15	  5.9%
Asn (N)  11	  4.3%
Asp (D)  13	  5.1%
Cys (C)   1	  0.4%
Gln (Q)  11	  4.3%
Glu (E)  16	  6.3%
Gly (G)  16	  6.3%
His (H)   6	  2.4%
Ile (I)  14	  5.5%
Leu (L)  22	  8.6%
Lys (K)  34	 13.3%
Met (M)   4	  1.6%
Phe (F)  11	  4.3%
Pro (P)   5	  2.0%
Ser (S)  16	  6.3%
Thr (T)  17	  6.7%
Trp (W)   2	  0.8%
Tyr (Y)   2	  0.8%
Val (V)  24	  9.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 49

Atomic composition:

Carbon      C	      1277
Hydrogen    H	      2110
Nitrogen    N	       370
Oxygen      O	       371
Sulfur      S	         5

Formula: C1277H2110N370O371S5
Total number of atoms: 4133

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13980
Abs 0.1% (=1 g/l)   0.486, assuming all pairs of Cys residues form cystines


Ext. coefficient    13980
Abs 0.1% (=1 g/l)   0.486, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.52
This classifies the protein as stable.



Aliphatic index: 88.24

Grand average of hydropathicity (GRAVY): -0.494