ProtParam
User-provided sequence:
10 20 30 40 50 60
MAVKHKSESL KHEEGAAKKA KTGLLKLKKI MDIESNVVKY SICIPTTVID NCNNLEQVTF
70 80 90 100 110 120
TAYQIARTAV LFNVQEIIVL DQSKDKKHEK KSRSKETISD CLLLATLLQY FVTPPNLLDT
130 140 150 160 170 180
TFKKKNKLYL KCASTFPPLN QLPFMNASAE QHYKEGLSIA RDSSKGKSDD ALTNLVYIGK
190 200 210 220 230 240
NQIITLSNQN IPNTARVTVD TERKEVVSPI DAYKGKPLGY HVRMASTLNE VSEGYTKIVW
250 260 270 280 290 300
VNSGDFHYDE ELSKYHKVET KLPYIAKLKK SSTSEKPCNI LLIFGKWGHL KRCFRRSDLE
310 320 330 340
SSSLHHYFSG QLQFPASVPQ GNIPIQDSLP IALTMFQRWA S
References and
documentation are available.
Number of amino acids: 341
Molecular weight: 38546.46
Theoretical pI: 9.33
Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 48
Atomic composition:
Carbon C 1730
Hydrogen H 2766
Nitrogen N 464
Oxygen O 508
Sulfur S 11
Formula: C
1730H
2766N
464O
508S
11
Total number of atoms: 5479
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36245
Abs 0.1% (=1 g/l) 0.940, assuming all pairs of Cys residues form cystines
Ext. coefficient 35870
Abs 0.1% (=1 g/l) 0.931, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.57
This classifies the protein as unstable.
Aliphatic index: 88.06
Grand average of hydropathicity (GRAVY): -0.396