ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAVLSVGFFA CRSHFPVLPL FYCFSKVQLN TVAAAMETVD DKDSDHHHDK GSNKTSKAPE 

        70         80         90        100        110        120 
WKKLNSKDLG ITTSMISKPT RIVLNGLKSK GYDVYLVGGC VRDLILKRTP KDFDILTSAE 

       130        140        150        160        170        180 
LREVVRSFSR CEIIGKKFPI CHVHIGNDMI EVSSFSTSAQ NSLRNTRTGS GKSNGSYDED 

       190        200        210        220        230        240 
SIRFNNCLQR DFTINGLMFD PYAKVIYDYL GGIEDIKKAK VRTVFHAGTS FQEDSARILR 

       250        260        270        280        290        300 
GTRIAARLGF TISKETAHFL KNLSFLVQRL HRGRILLEMN YMLAYGSAEA SLRLLWKFGI 

       310        320        330        340        350        360 
LEILLPIQAA YLVRTGFKRR DKRSNLLLSL FGNLDKLLAP DKPCHSSLWL TILALHKALA 

       370        380        390        400        410        420 
DQPRYPSVVA AFSLAVHNGG DVLEAVKNTR KVTKPHNRSF FELLEPEEMD SQTLLDEVMD 

       430        440        450        460        470        480 
FDSSIKEALG QMTDGRFISK AMAAYPQAPY SDMVFIPLQL YLDARRIFEC VKENGQKGFV 

       490        500        510        520        530        540 
PKQDSKREPE DDLETKPILK KHKENSEEEN KEQIKGTVEL LETKSDQAPV KETVEGLDET 

       550        560        570        580        590        600 
PDSVEVPKKY MSGLLIRCFP SRKKTLYVCC LPRDTKMPDI IDFFKDVGQV VSVQLTTKRN 

       610        620        630        640        650        660 
GLRLSTGFVE FASANEAKKA LDMKNGEYLC GNKLILGMAS CENIAQPKYE DYIQQESLPI 

       670        680        690        700        710        720 
EEEETPPKIV QSGANTLGCS IQAAAFSMTD ILSFHFYRLD FFNDVGEVVS VRLIVSPESK 

       730        740        750        760        770        780 
HVGYGFVEFA SPCLANMALE KKNGEYLHDH KIFLGVAKTA PYPPRIKYNL AEKLWYEDYL 

       790        800        810        820        830        840 
LRDSLLIEED ETVEGLDETP SCVEAVALRE KVLIIAHVPR RTKISHIIDF FKDAGQVVNV 

       850        860        870        880 
RLIVDQKGKP FGRGFVEFTS ADEAKKVRLV YSNDEKYFTS H 

References and documentation are available.

Number of amino acids: 881

Molecular weight: 99377.22

Theoretical pI: 7.53

Amino acid composition: 
Ala (A)  57	  6.5%
Arg (R)  46	  5.2%
Asn (N)  33	  3.7%
Asp (D)  55	  6.2%
Cys (C)  16	  1.8%
Gln (Q)  24	  2.7%
Glu (E)  63	  7.2%
Gly (G)  50	  5.7%
His (H)  21	  2.4%
Ile (I)  49	  5.6%
Leu (L)  91	 10.3%
Lys (K)  73	  8.3%
Met (M)  18	  2.0%
Phe (F)  47	  5.3%
Pro (P)  38	  4.3%
Ser (S)  65	  7.4%
Thr (T)  42	  4.8%
Trp (W)   4	  0.5%
Tyr (Y)  28	  3.2%
Val (V)  61	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 118
Total number of positively charged residues (Arg + Lys): 119

Atomic composition:

Carbon      C	      4453
Hydrogen    H	      7049
Nitrogen    N	      1195
Oxygen      O	      1310
Sulfur      S	        34

Formula: C4453H7049N1195O1310S34
Total number of atoms: 14041

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    64720
Abs 0.1% (=1 g/l)   0.651, assuming all pairs of Cys residues form cystines


Ext. coefficient    63720
Abs 0.1% (=1 g/l)   0.641, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.19
This classifies the protein as unstable.



Aliphatic index: 88.52

Grand average of hydropathicity (GRAVY): -0.275