ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAVNPELAPF TLSRGIPSFD DQALSTIIQL QDCIQQAIQQ LNYSTAEFLA ELLYAECSIL 

        70         80         90        100        110        120 
DKSSVYWSDA VYLYALSLFL NKSYHTAFQI SKEFKEYHLG IAYIFGRCAL QLSQGVNEAI 

       130        140        150        160        170        180 
LTLLSIINVF SSNSSNTRIN MVLNSNLVHI PDLATLNCLL GNLYMKLDHS KEGAFYHSEA 

       190        200        210        220        230        240 
LAINPYLWES YEAICKMRAT VDLKRVFFDI AGKKSNSHNN NAASSFPSTS LSHFEPRSQP 

       250        260        270        280        290        300 
SLYSKTNKNG NNNINNNVNT LFQSSNSPPS TSASSFSSIQ HFSRSQQQQA NTSIRTCQNK 

       310        320        330        340        350        360 
NTQTPKNPAI NSKTSSALPN NISMNLVSPS SKQPTISSLA KVYNRNKLLT TPPSKLLNND 

       370        380        390        400        410        420 
RNHQNNNNNN NNNNNNNNNN NNNNNNNNII NKTTFKTPRN LYSSTGRLTT SKKNPRSLII 

       430        440        450        460        470        480 
SNSILTSDYQ ITLPEIMYNF ALILRSSSQY NSFKAIRLFE SQIPSHIKDT MPWCLVQLGK 

       490        500        510        520        530        540 
LHFEIINYDM SLKYFNRLKD LQPARVKDME IFSTLLWHLH DKVKSSNLAN GLMDTMPNKP 

       550        560        570        580        590        600 
ETWCCIGNLL SLQKDHDAAI KAFEKATQLD PNFAYAYTLQ GHEHSSNDSS DSAKTCYRKA 

       610        620        630        640        650        660 
LACDPQHYNA YYGLGTSAMK LGQYEEALLY FEKARSINPV NVVLICCCGG SLEKLGYKEK 

       670        680        690        700        710        720 
ALQYYELACH LQPTSSLSKY KMGQLLYSMT RYNVALQTFE ELVKLVPDDA TAHYLLGQTY 

       730        740        750 
RIVGRKKDAI KELTVAMNLD PKGNQVIIDE LQKCHMQE 

References and documentation are available.

Number of amino acids: 758

Molecular weight: 85436.70

Theoretical pI: 8.88

Amino acid composition: 
Ala (A)  51	  6.7%
Arg (R)  22	  2.9%
Asn (N)  84	 11.1%
Asp (D)  28	  3.7%
Cys (C)  16	  2.1%
Gln (Q)  39	  5.1%
Glu (E)  30	  4.0%
Gly (G)  23	  3.0%
His (H)  20	  2.6%
Ile (I)  45	  5.9%
Leu (L)  86	 11.3%
Lys (K)  50	  6.6%
Met (M)  16	  2.1%
Phe (F)  27	  3.6%
Pro (P)  31	  4.1%
Ser (S)  83	 10.9%
Thr (T)  42	  5.5%
Trp (W)   5	  0.7%
Tyr (Y)  36	  4.7%
Val (V)  24	  3.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 72

Atomic composition:

Carbon      C	      3772
Hydrogen    H	      5920
Nitrogen    N	      1042
Oxygen      O	      1159
Sulfur      S	        32

Formula: C3772H5920N1042O1159S32
Total number of atoms: 11925

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    82140
Abs 0.1% (=1 g/l)   0.961, assuming all pairs of Cys residues form cystines


Ext. coefficient    81140
Abs 0.1% (=1 g/l)   0.950, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.09
This classifies the protein as unstable.



Aliphatic index: 83.31

Grand average of hydropathicity (GRAVY): -0.435