ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAVPCMELNN KMKMPVLGLG TWQAPPGKVE EVVKHAIDAG YRHIDCAYFY QNEHEIGNAI 

        70         80         90        100        110        120 
KQKIKEGAVK REDLFVVTKL WNTFHEKSLV KEGCKRSLTA LQLDYVDLYL MHYPMGFKAG 

       130        140        150        160        170        180 
EELLPEDDKG MIIPSDTDFL DTWEAMEELV DCGKVKAIGI SNFNHEQIER LLNKPGLKYK 

       190        200        210        220        230        240 
PVVNQIECHP YLTQEKLIKY CHSKGIAVTA YSPLGSPNRP WAKPGEPMLL EDPKIKEIAA 

       250        260        270        280        290        300 
RYHKTPAQVL IRFIIQRNLA VIPKSDKQQR IKENMQVFDF ELSKKEMDVI LSFNRNWRAI 

       310 
PVPQSANHKD YPFNAEY 

References and documentation are available.

Number of amino acids: 317

Molecular weight: 36472.29

Theoretical pI: 7.63

Amino acid composition: 
Ala (A)  21	  6.6%
Arg (R)  11	  3.5%
Asn (N)  16	  5.0%
Asp (D)  16	  5.0%
Cys (C)   6	  1.9%
Gln (Q)  13	  4.1%
Glu (E)  26	  8.2%
Gly (G)  16	  5.0%
His (H)  10	  3.2%
Ile (I)  22	  6.9%
Leu (L)  27	  8.5%
Lys (K)  32	 10.1%
Met (M)  11	  3.5%
Phe (F)  11	  3.5%
Pro (P)  21	  6.6%
Ser (S)  11	  3.5%
Thr (T)   9	  2.8%
Trp (W)   5	  1.6%
Tyr (Y)  13	  4.1%
Val (V)  20	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 43

Atomic composition:

Carbon      C	      1648
Hydrogen    H	      2582
Nitrogen    N	       436
Oxygen      O	       464
Sulfur      S	        17

Formula: C1648H2582N436O464S17
Total number of atoms: 5147

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    47245
Abs 0.1% (=1 g/l)   1.295, assuming all pairs of Cys residues form cystines


Ext. coefficient    46870
Abs 0.1% (=1 g/l)   1.285, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.62
This classifies the protein as unstable.



Aliphatic index: 85.21

Grand average of hydropathicity (GRAVY): -0.445