ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAVPVLGGFP IFMVVRVLGF IIAALVLTWT VHYRGGLALS SDNKDHIFNV HPVMMVIGLI 

        70         80         90        100        110        120 
LFNGEAMLAY KSVQGTKNLK KLVHLTLQLT AFILSLIGVW AALKFHIDKG IENFYSLHSW 

       130        140        150        160        170        180 
LGLACLFLFA FQWAAGFVTY WYPGGSRNSR ASLMPWHVFL GISIYALALV TATTGILEKV 

       190        200        210        220        230 
TFLQVNQVIT RYSTEAMLVN TMGVLILILG GFVILGVVTP VSGKDQVLTQ 

References and documentation are available.

Number of amino acids: 230

Molecular weight: 25289.21

Theoretical pI: 9.55

Amino acid composition: 
Ala (A)  18	  7.8%
Arg (R)   5	  2.2%
Asn (N)   8	  3.5%
Asp (D)   4	  1.7%
Cys (C)   1	  0.4%
Gln (Q)   7	  3.0%
Glu (E)   4	  1.7%
Gly (G)  21	  9.1%
His (H)   7	  3.0%
Ile (I)  17	  7.4%
Leu (L)  34	 14.8%
Lys (K)   9	  3.9%
Met (M)   8	  3.5%
Phe (F)  15	  6.5%
Pro (P)   6	  2.6%
Ser (S)  12	  5.2%
Thr (T)  15	  6.5%
Trp (W)   6	  2.6%
Tyr (Y)   7	  3.0%
Val (V)  26	 11.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 8
Total number of positively charged residues (Arg + Lys): 14

Atomic composition:

Carbon      C	      1194
Hydrogen    H	      1861
Nitrogen    N	       289
Oxygen      O	       296
Sulfur      S	         9

Formula: C1194H1861N289O296S9
Total number of atoms: 3649

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43430
Abs 0.1% (=1 g/l)   1.717, assuming all pairs of Cys residues form cystines


Ext. coefficient    43430
Abs 0.1% (=1 g/l)   1.717, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.57
This classifies the protein as stable.



Aliphatic index: 127.09

Grand average of hydropathicity (GRAVY): 0.843