ProtParam
User-provided sequence:
10 20 30 40 50 60
MAVSARLRTT AEDTSIAKST QDPIGDTEIS VANAKGSSDS NIKNSPGGNS VGQESELEHV
70 80 90 100 110 120
PEEDDSGDKE ADHEDSETAT AKKRKAQPLK NPKKSLKRGR VPAPLNLSDS NTNTHGGNIK
130 140 150 160 170 180
DGNLASSNSA HFPPVANQNV KSAPAQVTQH SKFQPRVQYL GKASSRQSIQ VNNSSNSYGK
190 200 210 220 230 240
PHMPSAGIMS AMNPYMPMNR YIMSPYYNPY GIPPPHMLNK PIMTPYVSYP YPMGPRTSIP
250 260 270 280 290 300
YAMQGGNARP YEENEYSASN YRNKRVNDSY DSPLSGTAST GKTRRSEEGS RNSSVGSSAN
310 320 330 340 350 360
AGPTQQRADL RPADMIPAEE YHFERDALLS ANTKARSAST STSTSTSTNR DRSSWHEAEP
370 380 390 400 410 420
NKDEEEGTDL AIEDGAVPTP TFTTFQRTSQ PQQQSPSLLQ GEIRLSSHIF AFEFPLSSSN
430 440 450
VDKKMFMSIC NKVWNESKEL TKKSSSHHRT GK
References and
documentation are available.
Number of amino acids: 452
Molecular weight: 49356.19
Theoretical pI: 8.62
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 52
Atomic composition:
Carbon C 2105
Hydrogen H 3318
Nitrogen N 630
Oxygen O 714
Sulfur S 15
Formula: C
2105H
3318N
630O
714S
15
Total number of atoms: 6782
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 34840
Abs 0.1% (=1 g/l) 0.706, assuming all pairs of Cys residues form cystines
Ext. coefficient 34840
Abs 0.1% (=1 g/l) 0.706, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 59.78
This classifies the protein as unstable.
Aliphatic index: 48.43
Grand average of hydropathicity (GRAVY): -0.982