ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAVSYNALAQ SLARSSCFIP KPYSFRDTKL RSRSNVVFAC NDNKNIALQA KVDNLLDRIK 

        70         80         90        100        110        120 
WDDKGLAVAI AQNVDTGAVL MQGFVNREAL STTISSRKAT FFSRSRSTLW TKGETSNNFI 

       130        140        150        160        170        180 
NILDVYVDCD RDSIIYLGTP DGPTCHTGEE TCYYTSVFDQ LNNDEASGNK LALTTLYSLE 

       190        200        210        220        230        240 
SIISKRKEES TVPQEGKPSW TRRLLTDDAL LCSKIREEAD ELCRTLEDNE EVSRTPSEMA 

       250        260        270        280 
DVLYHAMVLL SKRGVKMEDV LEVLRKRFSQ SGIEEKQNRT K 

References and documentation are available.

Number of amino acids: 281

Molecular weight: 31667.75

Theoretical pI: 5.89

Amino acid composition: 
Ala (A)  19	  6.8%
Arg (R)  20	  7.1%
Asn (N)  16	  5.7%
Asp (D)  20	  7.1%
Cys (C)   7	  2.5%
Gln (Q)   8	  2.8%
Glu (E)  20	  7.1%
Gly (G)  11	  3.9%
His (H)   2	  0.7%
Ile (I)  13	  4.6%
Leu (L)  28	 10.0%
Lys (K)  18	  6.4%
Met (M)   5	  1.8%
Phe (F)   9	  3.2%
Pro (P)   7	  2.5%
Ser (S)  28	 10.0%
Thr (T)  21	  7.5%
Trp (W)   3	  1.1%
Tyr (Y)   8	  2.8%
Val (V)  18	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      1374
Hydrogen    H	      2212
Nitrogen    N	       390
Oxygen      O	       443
Sulfur      S	        12

Formula: C1374H2212N390O443S12
Total number of atoms: 4431

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    28795
Abs 0.1% (=1 g/l)   0.909, assuming all pairs of Cys residues form cystines


Ext. coefficient    28420
Abs 0.1% (=1 g/l)   0.897, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.99
This classifies the protein as unstable.



Aliphatic index: 82.24

Grand average of hydropathicity (GRAVY): -0.461