ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAYADSPENA IAVIKQRTAL MNRCLSQHKL MESLQHTSIM LTELRNPNLS PKKYYELYVI 

        70         80         90        100        110        120 
IFDSLTNLST YLIENHPQNH HLADLYELVQ YTGNVVPRLY LMITVGTSYL TFNEAPKKEI 

       130        140        150        160        170        180 
LKDMIEMCRG VQNPIRGLFL RYYLSQRTKE LLPEDDPSFN SQFIMNNFIE MNKLWVRLQH 

       190        200        210        220        230        240 
QGPLRERETR TRERKELQIL VGSQLVRLSQ IIDDNFQMYK QDILPTILEQ VIQCRDLVSQ 

       250        260        270        280        290        300 
EYLLDVICQV FADEFHLKTL DTLLQTTLHL NPDVSINKIV LTLVDRLNDY VTRQLEDDPN 

       310        320        330        340        350        360 
ATSTNAYLDM DVFGTFWDYL TVLNHERPDL SLQQFIPLVE SVIVLSLKWY PNNFDNLNKL 

       370        380        390        400        410        420 
FELVLQKTKD YGQKNISLES EHLFLVLLSF QNSKLQLTSS TTAPPNSPVT SKKHFIFQLI 

       430        440        450        460        470        480 
SQCQAYKNIL ALQSISLQKK VVNEIIDILM DREVEEMADN DSESKLHPPG HSAYLVIEDK 

       490        500        510        520        530        540 
LQVQRLLSIC EPLIISRSGP PANVASSDTN VDEVFFNRHD EEESWILDPI QEKLAHLIHW 

       550        560        570        580        590        600 
IMNTTSRKQT MKNKIQFSLE AQLEILLLIK SSFIKGGINV KYTFPAIITN FWKLMRKCRM 

       610        620        630        640        650        660 
IQEYLLKKRP DNKTLLSHYS NLLKQMFKFV SRCINDIFNS CNNSCTDLIL KLNLQCAILA 

       670        680        690        700        710        720 
DQLQLNEISY DFFSQAFTIF EESLSDSKTQ LQALIYIAQS LQKTRSLYKE AYYDSLIVRC 

       730        740        750        760        770        780 
TLHGSKLLKK QDQCRAVYLC SHLWWATEIS NIGEEEGITD NFYRDGKRVL ECLQRSLRVA 

       790        800        810        820        830        840 
DSIMDNEQSC ELMVEILNRC LYYFIHGDES ETHISIKYIN GLIELIKTNL KSLKLEDNSA 

       850        860        870        880        890        900 
SMITNSISDL HITGENNVKA SSNADDGSVI TDKESNVAIG SDGTYIQLNT LNGSSTLIRG 

       910        920        930        940 
VVATASGSKL LHQLKYIPIH HFRRTCEYIE SQREVDDRFK VIYV 

References and documentation are available.

Number of amino acids: 944

Molecular weight: 109142.32

Theoretical pI: 5.87

Amino acid composition: 
Ala (A)  35	  3.7%
Arg (R)  41	  4.3%
Asn (N)  60	  6.4%
Asp (D)  54	  5.7%
Cys (C)  18	  1.9%
Gln (Q)  55	  5.8%
Glu (E)  61	  6.5%
Gly (G)  25	  2.6%
His (H)  25	  2.6%
Ile (I)  78	  8.3%
Leu (L) 126	 13.3%
Lys (K)  57	  6.0%
Met (M)  21	  2.2%
Phe (F)  35	  3.7%
Pro (P)  28	  3.0%
Ser (S)  79	  8.4%
Thr (T)  52	  5.5%
Trp (W)   8	  0.8%
Tyr (Y)  37	  3.9%
Val (V)  49	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 115
Total number of positively charged residues (Arg + Lys): 98

Atomic composition:

Carbon      C	      4878
Hydrogen    H	      7747
Nitrogen    N	      1297
Oxygen      O	      1458
Sulfur      S	        39

Formula: C4878H7747N1297O1458S39
Total number of atoms: 15419

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   100255
Abs 0.1% (=1 g/l)   0.919, assuming all pairs of Cys residues form cystines


Ext. coefficient    99130
Abs 0.1% (=1 g/l)   0.908, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.71
This classifies the protein as unstable.



Aliphatic index: 103.04

Grand average of hydropathicity (GRAVY): -0.233