ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MAYSDYSDGA DDMPDFHDEG EFDDYLNDDE YDLMNEVFPT LKAQLQDYQG WDNLSLKLAL 

        70         80         90        100        110        120 
FDNNFDLEST LAELKKTLKK KKTPKKPIAA ANGSANVTQK LANISISQQR PNDRLPDWLD 

       130        140        150        160        170        180 
EEESEGERNG EEANDEKTVQ RYYKTTVPTK PKKPHDISAF VKSALPHLSF VVLGHVDAGK 

       190        200        210        220        230        240 
STLMGRLLYD LNIVNQSQLR KLQRESETMG KSSFKFAWIM DQTNEERERG VTVSICTSHF 

       250        260        270        280        290        300 
STHRANFTIV DAPGHRDFVP NAIMGISQAD MAILCVDCST NAFESGFDLD GQTKEHMLLA 

       310        320        330        340        350        360 
SSLGIHNLII AMNKMDNVDW SQQRFEEIKS KLLPYLVDIG FFEDNINWVP ISGFSGEGVY 

       370        380        390        400        410        420 
KIEYTDEVRQ WYNGPNLMST LENAAFKISK ENEGINKDDP FLFSVLEIIP SKKTSNDLAL 

       430        440        450        460        470        480 
VSGKLESGSI QPGESLTIYP SEQSCIVDKI QVGSQQGQST NHEETDVAIK GDFVTLKLRK 

       490        500        510        520        530        540 
AYPEDIQNGD LAASVDYSSI HSAQCFVLEL TTFDMNRPLL PGTPFILFIG VKEQPARIKR 

       550        560        570        580        590        600 
LISFIDKGNT ASKKKIRHLG SKQRAFVEIE LIEVKRWIPL LTAHENDRLG RVVLRKDGRT 

       610 
IAAGKISEIT Q 

References and documentation are available.

Number of amino acids: 611

Molecular weight: 68729.59

Theoretical pI: 5.20

Amino acid composition: 
Ala (A)  37	  6.1%
Arg (R)  24	  3.9%
Asn (N)  33	  5.4%
Asp (D)  47	  7.7%
Cys (C)   5	  0.8%
Gln (Q)  27	  4.4%
Glu (E)  44	  7.2%
Gly (G)  36	  5.9%
His (H)  13	  2.1%
Ile (I)  42	  6.9%
Leu (L)  56	  9.2%
Lys (K)  46	  7.5%
Met (M)  13	  2.1%
Phe (F)  28	  4.6%
Pro (P)  25	  4.1%
Ser (S)  49	  8.0%
Thr (T)  32	  5.2%
Trp (W)   7	  1.1%
Tyr (Y)  15	  2.5%
Val (V)  32	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 91
Total number of positively charged residues (Arg + Lys): 70

Atomic composition:

Carbon      C	      3048
Hydrogen    H	      4792
Nitrogen    N	       822
Oxygen      O	       950
Sulfur      S	        18

Formula: C3048H4792N822O950S18
Total number of atoms: 9630

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    61100
Abs 0.1% (=1 g/l)   0.889, assuming all pairs of Cys residues form cystines


Ext. coefficient    60850
Abs 0.1% (=1 g/l)   0.885, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.45
This classifies the protein as stable.



Aliphatic index: 83.80

Grand average of hydropathicity (GRAVY): -0.460