ProtParam
User-provided sequence:
10 20 30 40 50 60
MAYVNEQNPQ FLLYNGMHNQ THSTPQYHNH HHHHHQSPTY PQSYFNPYSH QSYQQHHLNS
70 80 90 100 110 120
DVNFQMVPQS TSVPPDPFCS IEPMETNVQA KEQILEEIVR ECEEIERRSN SSASPASNWS
130 140 150 160 170 180
SDEHDSQSEK SYHPYKTPEK KERKKAQNRL AATRYREKKR REKEEAMTCI EGLSVTNGKL
190 200
KDQVSELERE IRYFKKFMTE MGMKTVTD
References and
documentation are available.
Number of amino acids: 208
Molecular weight: 24573.14
Theoretical pI: 6.53
Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 26
Atomic composition:
Carbon C 1061
Hydrogen H 1626
Nitrogen N 314
Oxygen O 340
Sulfur S 11
Formula: C
1061H
1626N
314O
340S
11
Total number of atoms: 3352
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 22015
Abs 0.1% (=1 g/l) 0.896, assuming all pairs of Cys residues form cystines
Ext. coefficient 21890
Abs 0.1% (=1 g/l) 0.891, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 65.49
This classifies the protein as unstable.
Aliphatic index: 42.64
Grand average of hydropathicity (GRAVY): -1.296