ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MCANIPEFDS FYENENINYN LESFAPLNCD VNSPFLPINN NDINVNAYGD ENLTYSNFLL 

        70         80         90        100        110        120 
SYNDKLATTT AKNNSINNSN SNNNSNNNKN NNNNHNNNNL LGNDISQMAF LLDYPSTLNE 

       130        140        150        160        170        180 
PQFAVNCKDI YRKDISTPSS LVSSLPPAKF SLSLSNSPSP PPPSSSSLKH GEAIISNTSE 

       190        200        210        220        230        240 
SSDIFADPNS FEKDTMPLTQ ELTLENLNNQ LNYPDFTINA IEQDPAPSSF SSSSSSSEST 

       250        260        270        280        290        300 
VSSSRKRKPC HDSYTHSSPS SSESKKISDS RLSAEGLAKV LNLESPEEAL KRERFILGIF 

       310        320        330        340        350        360 
QNELNYPLGY KTWIRDTTKE YRTKLINQLH ERVKVKYPEY NQSILETIIR RGTYYMMQSR 

       370 
LRRERRMKLK ERKRTT 

References and documentation are available.

Number of amino acids: 376

Molecular weight: 42604.04

Theoretical pI: 6.05

Amino acid composition: 
Ala (A)  15	  4.0%
Arg (R)  18	  4.8%
Asn (N)  51	 13.6%
Asp (D)  17	  4.5%
Cys (C)   4	  1.1%
Gln (Q)   9	  2.4%
Glu (E)  26	  6.9%
Gly (G)   7	  1.9%
His (H)   5	  1.3%
Ile (I)  21	  5.6%
Leu (L)  35	  9.3%
Lys (K)  22	  5.9%
Met (M)   6	  1.6%
Phe (F)  14	  3.7%
Pro (P)  24	  6.4%
Ser (S)  56	 14.9%
Thr (T)  21	  5.6%
Trp (W)   1	  0.3%
Tyr (Y)  16	  4.3%
Val (V)   8	  2.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      1847
Hydrogen    H	      2895
Nitrogen    N	       523
Oxygen      O	       616
Sulfur      S	        10

Formula: C1847H2895N523O616S10
Total number of atoms: 5891

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    29590
Abs 0.1% (=1 g/l)   0.695, assuming all pairs of Cys residues form cystines


Ext. coefficient    29340
Abs 0.1% (=1 g/l)   0.689, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 59.00
This classifies the protein as unstable.



Aliphatic index: 68.24

Grand average of hydropathicity (GRAVY): -0.843