ProtParam
User-provided sequence:
10 20 30 40 50 60
MCASPSTAPG FFNPRPQSGA ELSAFDIGLS RSMGLGVPPH SAWHEPPASL GGHLHAASAG
70 80 90 100 110 120
PGTTTGSVAT GGGGTTPSSV ASQQSAVIKQ DLSCPSLNQA GSGHHPGIKE DLSSSLPSAN
130 140 150 160 170 180
GGSAGGHHSG SGSGSGSGVN PGHGSDMLPL IKGHGQDMLT SIKGQPTGCG STTPSSQANS
190 200 210 220 230 240
SHSQSSNSGS QIDSKQNIEC VVCGDKSSGK HYGQFTCEGC KSFFKRSVRR NLTYSCRGSR
250 260 270 280 290 300
NCPIDQHHRN QCQYCRLKKC LKMGMRREAV QRGRVPPTQP GLAGMHGQYQ IANGDPMGIA
310 320 330 340 350 360
GFNGHSYLSS YISLLLRAEP YPTSRYGQCM QPNNIMGIDN ICELAARLLF SAVEWAKNIP
370 380 390 400 410 420
FFPELQVTDQ VALLRLVWSE LFVLNASQCS MPLHVAPLLA AAGLHASPMA ADRVVAFMDH
430 440 450 460 470 480
IRIFQEQVEK LKALHVDSAE YSCLKAIVLF TTDACGLSDV THIESLQEKS QCALEEYCRT
490 500 510 520 530 540
QYPNQPTRFG KLLLRLPSLR TVSSQVIEQL FFVRLVGKTP IETLIRDMLL SGNSFSWPYL
PSM
References and
documentation are available.
Number of amino acids: 543
Molecular weight: 57987.75
Theoretical pI: 8.55
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 44
Atomic composition:
Carbon C 2518
Hydrogen H 3974
Nitrogen N 734
Oxygen O 773
Sulfur S 34
Formula: C
2518H
3974N
734O
773S
34
Total number of atoms: 8033
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 41005
Abs 0.1% (=1 g/l) 0.707, assuming all pairs of Cys residues form cystines
Ext. coefficient 39880
Abs 0.1% (=1 g/l) 0.688, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 52.39
This classifies the protein as unstable.
Aliphatic index: 73.68
Grand average of hydropathicity (GRAVY): -0.242