ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MCDKSKTVLD YTIEFLDKYI PEWFETGNKC PLFIFFSGPQ GSGKSFTSIQ IYNHLMEKYG 

        70         80         90        100        110        120 
GEKSIGYASI DDFYLTHEDQ LKLNEQFKNN KLLQGRGLPG THDMKLLQEV LNTIFNNNEH 

       130        140        150        160        170        180 
PDQDTVVLPK YDKSQFKGEG DRCPTGQKIK LPVDIFILEG WFLGFNPILQ GIENNDLLTG 

       190        200        210        220        230        240 
DMVDVNAKLF FYSDLLWRNP EIKSLGIVFT TDNINNVYGW RLQQEHELIS KVGKGMTDEQ 

       250        260        270        280        290 
VHAFVDRYMP SYKLYLNDFV RSESLGSIAT LTLGIDSNRN VYSTKTRCIE 

References and documentation are available.

Number of amino acids: 290

Molecular weight: 33336.83

Theoretical pI: 5.23

Amino acid composition: 
Ala (A)   4	  1.4%
Arg (R)   8	  2.8%
Asn (N)  20	  6.9%
Asp (D)  21	  7.2%
Cys (C)   4	  1.4%
Gln (Q)  13	  4.5%
Glu (E)  18	  6.2%
Gly (G)  23	  7.9%
His (H)   6	  2.1%
Ile (I)  20	  6.9%
Leu (L)  31	 10.7%
Lys (K)  22	  7.6%
Met (M)   6	  2.1%
Phe (F)  18	  6.2%
Pro (P)  11	  3.8%
Ser (S)  17	  5.9%
Thr (T)  17	  5.9%
Trp (W)   4	  1.4%
Tyr (Y)  13	  4.5%
Val (V)  14	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1508
Hydrogen    H	      2309
Nitrogen    N	       385
Oxygen      O	       449
Sulfur      S	        10

Formula: C1508H2309N385O449S10
Total number of atoms: 4661

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    41620
Abs 0.1% (=1 g/l)   1.248, assuming all pairs of Cys residues form cystines


Ext. coefficient    41370
Abs 0.1% (=1 g/l)   1.241, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.02
This classifies the protein as stable.



Aliphatic index: 83.97

Grand average of hydropathicity (GRAVY): -0.414