ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MCFLLETSAS PRSKLSKDFK PQFTLLSSVT KKKKKKVRPH NFQCIHSLNF VYFLFIHSFL 

        70         80         90        100        110        120 
FEYNQLLVLP LNKNLPSLNF SRNSSMKLSA LLALSASTAV LAAPAVHHSD NHHHNDKRAV 

       130        140        150        160        170        180 
VTVTQYVNAD GAVVIPAATT ATSAAADGKV ESVAAATTTL SSTAAAATTS AAASSSSSSS 

       190        200        210        220        230        240 
SSSSSSSSVG SGDFEDGTIS CSDFPSGQGA VSLDWLGLGG WASIMDMNGN TATSCQDGYY 

       250        260        270        280        290        300 
CSYACSPGYA KTQWPSEQPS DGRSVGGLYC KNGKLYRSNT DTNSLCVEGQ GSAQAVNKVS 

       310        320        330        340        350        360 
GSIAICGTDY PGSENMVVPT VVGAGSSQPI NVIKEDSYYQ WQGKKTSAQY YVNNAGVSVE 

       370        380        390        400        410        420 
DGCIWGTEGS GVGNWAPVVL GAGYTDGITY LSIIPNPNNK EAPNFNIKIV ATDGSTVNGA 

       430        440        450 
CSYENGVYSG SGSDGCTVSV TSGSANFVFY 

References and documentation are available.

Number of amino acids: 450

Molecular weight: 46921.02

Theoretical pI: 6.24

Amino acid composition: 
Ala (A)  44	  9.8%
Arg (R)   6	  1.3%
Asn (N)  29	  6.4%
Asp (D)  19	  4.2%
Cys (C)  12	  2.7%
Gln (Q)  14	  3.1%
Glu (E)  12	  2.7%
Gly (G)  41	  9.1%
His (H)   8	  1.8%
Ile (I)  15	  3.3%
Leu (L)  28	  6.2%
Lys (K)  22	  4.9%
Met (M)   5	  1.1%
Phe (F)  15	  3.3%
Pro (P)  18	  4.0%
Ser (S)  69	 15.3%
Thr (T)  31	  6.9%
Trp (W)   6	  1.3%
Tyr (Y)  19	  4.2%
Val (V)  37	  8.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 28

Atomic composition:

Carbon      C	      2049
Hydrogen    H	      3167
Nitrogen    N	       555
Oxygen      O	       675
Sulfur      S	        17

Formula: C2049H3167N555O675S17
Total number of atoms: 6463

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    62060
Abs 0.1% (=1 g/l)   1.323, assuming all pairs of Cys residues form cystines


Ext. coefficient    61310
Abs 0.1% (=1 g/l)   1.307, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.37
This classifies the protein as stable.



Aliphatic index: 70.89

Grand average of hydropathicity (GRAVY): -0.132