ProtParam
User-provided sequence:
10 20 30 40 50 60
MCGILGIVLA NQTTPVAPEL CDGCIFLQHR GQDAAGIATC GSRGRIYQCK GNGMARDVFT
70 80 90 100 110 120
QQRVSGLAGS MGIAHLRYPT AGSSANSEAQ PFYVNSPYGI NLAHNGNLVN TASLKRYMDE
130 140 150 160 170 180
DVHRHINTDS DSELLLNIFA AELEKHNKYR VNNEDVFHAL EGVYRLCRGG YACVGLLAGF
190 200 210 220 230 240
ALFGFRDPNG IRPLLFGERE NPDGTKDYML ASESVVFKAH NFTKYRDLKP GEAVIIPKNC
250 260 270 280 290 300
SKGEPEFKQV VPINSYRPDL FEYVYFARPD SVLDGISVYH TRLAMGSKLA ENILKQLKPE
310 320 330 340 350 360
DIDVVIPVPD TARTCALECA NVLGKPYREG FVKNRYVGRT FIMPNQRERV SSVRRKLNPM
370 380 390 400 410 420
ESEFKGKKVL IVDDSIVRGT TSKEIVNMAK ESGATKVYFA SAAPAIRYNH IYGIDLTDTK
430 440 450 460 470 480
NLIAYNRTDE EVAEVIGCER VIYQSLEDLI DCCKTDKITK FEDGVFTGNY VTGVEDGYIQ
490 500 510
ELEEKRESIA NNSSDMKAEV DIGLYNCADY
References and
documentation are available.
Number of amino acids: 510
Molecular weight: 56719.37
Theoretical pI: 5.90
Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 60
Atomic composition:
Carbon C 2500
Hydrogen H 3933
Nitrogen N 695
Oxygen O 764
Sulfur S 24
Formula: C
2500H
3933N
695O
764S
24
Total number of atoms: 7916
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 38125
Abs 0.1% (=1 g/l) 0.672, assuming all pairs of Cys residues form cystines
Ext. coefficient 37250
Abs 0.1% (=1 g/l) 0.657, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 28.96
This classifies the protein as stable.
Aliphatic index: 83.35
Grand average of hydropathicity (GRAVY): -0.310