ProtParam
User-provided sequence:
10 20 30 40 50 60
MCLEPISLVV FGSLVFFFGL VKYFKRGERQ RTRGILQPEY KDKYYYSKEK GEEMGEVANV
70 80 90 100 110 120
NEIPVKIRNH KYPAKEHNLR VKDLLLNRNP KLSKISTAFF IAGEELEGNK YCDTNKDFRQ
130 140 150 160 170 180
NRYFYHLSGV DIPASAILFN CSTDKLTLFL PNIDEEDVIW SGMPLSLDEA MRVFDIDEAL
190 200 210 220 230 240
YISDLGKKFK ELQDFAIFTT DLDNVHDENI ARSLIPSDPN FFYAMDETRA IKDWYEIESI
250 260 270 280 290 300
RKACQISDKS HLAVMSALPI ELNELQIQAE FEYHATRQGG RSLGYDPICC SGPACGTLHY
310 320 330 340 350 360
VKNSEDIKGK HSILIDAGAE WRQYTSDITR CFPTSGKFTA EHREVYETVL DMQNQAMERI
370 380 390 400 410 420
KPGAKWDDLH ALTHKVLIKH FLSMGIFKKE FSEDEIFKRR ASCAFYPHGL GHMLGLDVHD
430 440 450 460 470 480
VGGNPNYDDP DPMFRYLRIR RPLKENMVIT NEPGCYFNQF LIKEFLEKHP ERLEVVDMSV
490 500 510 520 530
LKRYMYVGGV RIEDDILVTK DGYENLTGIT SDPDEIEKIV QKGLKKPRSG FHVIV
References and
documentation are available.
Number of amino acids: 535
Molecular weight: 61753.56
Theoretical pI: 5.78
Total number of negatively charged residues (Asp + Glu): 83
Total number of positively charged residues (Arg + Lys): 70
Atomic composition:
Carbon C 2777
Hydrogen H 4297
Nitrogen N 737
Oxygen O 811
Sulfur S 24
Formula: C
2777H
4297N
737O
811S
24
Total number of atoms: 8646
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 58385
Abs 0.1% (=1 g/l) 0.945, assuming all pairs of Cys residues form cystines
Ext. coefficient 57760
Abs 0.1% (=1 g/l) 0.935, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 38.86
This classifies the protein as stable.
Aliphatic index: 83.27
Grand average of hydropathicity (GRAVY): -0.424