ProtParam
User-provided sequence:
10 20 30 40 50 60
MCNTNMSVST EGAAGTSQIP ASEQETLIIF YIGQYIMTKR LYDEKQQHIV YCSNDLLGDV
70 80 90 100 110 120
FGVPSFSVKE HRKIYAMIYR NLVVVSQQDS GTSPSESRCQ PEGGSDLKDP VQASQEEKPS
130 140 150 160 170 180
SSDVVSRPST SSRRRAISET EENTDELPGE RQRKRHRALS FDESLGLCVL REICCERSSS
190 200 210 220 230 240
SEATDTPSHQ DLDDGVSDHS ADCLDQDSVS DQFSVEFEVE SLDSEDYSLS DEGHELSDED
250 260 270 280 290 300
DEVYRVTVYQ AGESDADSFE GDPEISLADY WKCTSCNEMN PPLPSHCNRC WTLRENWLPD
310 320 330 340 350 360
DKGKDKVEIS EKAKLESSDQ AEEGLDVPDG KKVTEDDAKE SSAEDSEEKV AQMLLSQESD
370 380 390 400 410 420
DYSQPSTSSS IVYSSQESGK ELKEDTQDKE ESMESSFSLN AIEPCVICQG RPKNGCIVHG
430 440 450
KTGHLMSCFT CAKKLKKRNK PCPVCRQPIQ MIVLTYFN
References and
documentation are available.
Number of amino acids: 458
Molecular weight: 51050.92
Theoretical pI: 4.49
Total number of negatively charged residues (Asp + Glu): 90
Total number of positively charged residues (Arg + Lys): 47
Atomic composition:
Carbon C 2159
Hydrogen H 3397
Nitrogen N 603
Oxygen O 774
Sulfur S 27
Formula: C
2159H
3397N
603O
774S
27
Total number of atoms: 6960
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36995
Abs 0.1% (=1 g/l) 0.725, assuming all pairs of Cys residues form cystines
Ext. coefficient 35870
Abs 0.1% (=1 g/l) 0.703, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 58.46
This classifies the protein as unstable.
Aliphatic index: 61.90
Grand average of hydropathicity (GRAVY): -0.794