ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MCSPTNFLYE PFSSDAVTQN YDQNLKCTKC GAYYSMACSL REQNVWTCLF CNQSNSNAEL 

        70         80         90        100        110        120 
PLVPSNTYTL TSAKKEILSR RTIMIIDAIC DPHELNYLVS ILCNNYITRQ QEPLSIITIQ 

       130        140        150        160        170        180 
QSGHVILHNA VNHRRDAVFS INEFMTKYNL DKLNASYFEK KISEINQESY WFDKSTQGSL 

       190        200        210        220        230        240 
RKLLREICKI ANKVNISSKR DKRCTGLALF VSSVLASQCS LSAYCHIVSF LNGPCTKGGG 

       250        260        270        280        290        300 
KVMSRERGES MRQNHHFESK SSQLQLSKSP TKFYKKMLEK FANQSLIYEF FIASLDQIGI 

       310        320        330        340        350        360 
LEMSPLITSS MAVSQFDSFN DERFAMSFQK YLNLRDHNAI YNCHSKIMTA KNAIVVKDFP 

       370        380        390        400        410        420 
KYSLNPKNLS LPLEISLGHN SAEAPIQFQT TFENQTEKYI RIETLLLPKA NRSFGAQNEI 

       430        440        450        460        470        480 
VFSMKKIASR IIDSFAYSSK HTKELMKQLF LLPNQIRGKD VDMVNLIQWC YHIYRSPILS 

       490        500        510        520        530        540 
VRNTSPDERY LFLHRIINAS KDTCLSLCKP FIWSYSDLKH DWIVLDVPLT RAQILQDDKT 

       550        560        570        580        590        600 
TICVDGGSYL VLRRGKLLEK EGRELCCKLL NDLQRFPQPL YVETKTGGSQ DRFLKSKIIP 

       610        620        630 
LDITDKETLG TEDMTFNEYF NLFTDLSGSK 

References and documentation are available.

Number of amino acids: 630

Molecular weight: 72307.14

Theoretical pI: 8.85

Amino acid composition: 
Ala (A)  28	  4.4%
Arg (R)  29	  4.6%
Asn (N)  40	  6.3%
Asp (D)  30	  4.8%
Cys (C)  20	  3.2%
Gln (Q)  30	  4.8%
Glu (E)  33	  5.2%
Gly (G)  21	  3.3%
His (H)  14	  2.2%
Ile (I)  47	  7.5%
Leu (L)  68	 10.8%
Lys (K)  48	  7.6%
Met (M)  15	  2.4%
Phe (F)  32	  5.1%
Pro (P)  22	  3.5%
Ser (S)  65	 10.3%
Thr (T)  34	  5.4%
Trp (W)   5	  0.8%
Tyr (Y)  25	  4.0%
Val (V)  24	  3.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 77

Atomic composition:

Carbon      C	      3221
Hydrogen    H	      5084
Nitrogen    N	       868
Oxygen      O	       951
Sulfur      S	        35

Formula: C3221H5084N868O951S35
Total number of atoms: 10159

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    66000
Abs 0.1% (=1 g/l)   0.913, assuming all pairs of Cys residues form cystines


Ext. coefficient    64750
Abs 0.1% (=1 g/l)   0.895, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.84
This classifies the protein as unstable.



Aliphatic index: 86.68

Grand average of hydropathicity (GRAVY): -0.310