ProtParam
User-provided sequence:
10 20 30 40 50 60
MCSTKVPNER TEKMNSGLIS TRHQVDPKKE LSRILRTDAA VKGIERKANS EKYLTLWPKA
70 80 90 100 110 120
VLEALDEAIK ENRWQSALKI FNLLRKQHWY EPRCKTYTKL FKVLGNCKQP DQASLLFEVM
130 140 150 160 170 180
LSEGLKPTID VYTSLISVYG KSELLDKAFS TLEYMKSVSD CKPDVFTFTV LISCCCKLGR
190 200 210 220 230 240
FDLVKSIVLE MSYLGVGCST VTYNTIIDGY GKAGMFEEME SVLADMIEDG DSLPDVCTLN
250 260 270 280 290 300
SIIGSYGNGR NMRKMESWYS RFQLMGVQPD ITTFNILILS FGKAGMYKKM CSVMDFMEKR
310 320 330 340 350 360
FFSLTTVTYN IVIETFGKAG RIEKMDDVFR KMKYQGVKPN SITYCSLVNA YSKAGLVVKI
370 380 390 400 410 420
DSVLRQIVNS DVVLDTPFFN CIINAYGQAG DLATMKELYI QMEERKCKPD KITFATMIKT
430 440
YTAHGIFDAV QELEKQMISS GENLDIL
References and
documentation are available.
Number of amino acids: 447
Molecular weight: 50691.06
Theoretical pI: 8.40
Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 58
Atomic composition:
Carbon C 2262
Hydrogen H 3608
Nitrogen N 580
Oxygen O 667
Sulfur S 34
Formula: C
2262H
3608N
580O
667S
34
Total number of atoms: 7151
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 51060
Abs 0.1% (=1 g/l) 1.007, assuming all pairs of Cys residues form cystines
Ext. coefficient 50310
Abs 0.1% (=1 g/l) 0.992, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.74
This classifies the protein as stable.
Aliphatic index: 87.63
Grand average of hydropathicity (GRAVY): -0.120