ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDADELELKG HLKKLKKEEL LRRKQLKESN LQKRELEYNN ASKNTSIYIS GLPTDKTTKE 

        70         80         90        100        110        120 
GLTEQFCKYG MIRTNRDGEP LCKLYVNDKG AFKGDALITY SKEESVTLAI EMMNESIFLG 

       130        140        150        160        170        180 
KQIRVERAQF QNKEGDNMHG KENDLKEFNG PEPPIKRLKK AKSEGEGEVI DYNDDESLAK 

       190        200        210        220        230        240 
ADRTVIFANV FNIYKSYTND DINDIQEDLL EGCEEIGQVD SISVSPNKGE ATVVFKNNKV 

       250        260        270        280 
ALQCCKIMTG RYFDGQKLLA FISGDENTSS TSDKNEDSEV EDDLI 

References and documentation are available.

Number of amino acids: 285

Molecular weight: 32284.20

Theoretical pI: 4.91

Amino acid composition: 
Ala (A)  13	  4.6%
Arg (R)  10	  3.5%
Asn (N)  21	  7.4%
Asp (D)  23	  8.1%
Cys (C)   5	  1.8%
Gln (Q)  10	  3.5%
Glu (E)  32	 11.2%
Gly (G)  19	  6.7%
His (H)   2	  0.7%
Ile (I)  18	  6.3%
Leu (L)  25	  8.8%
Lys (K)  32	 11.2%
Met (M)   6	  2.1%
Phe (F)  10	  3.5%
Pro (P)   6	  2.1%
Ser (S)  18	  6.3%
Thr (T)  14	  4.9%
Trp (W)   0	  0.0%
Tyr (Y)   9	  3.2%
Val (V)  12	  4.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 42

Atomic composition:

Carbon      C	      1401
Hydrogen    H	      2248
Nitrogen    N	       382
Oxygen      O	       468
Sulfur      S	        11

Formula: C1401H2248N382O468S11
Total number of atoms: 4510

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13660
Abs 0.1% (=1 g/l)   0.423, assuming all pairs of Cys residues form cystines


Ext. coefficient    13410
Abs 0.1% (=1 g/l)   0.415, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.69
This classifies the protein as stable.



Aliphatic index: 75.61

Grand average of hydropathicity (GRAVY): -0.802