ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDADRGGGQE TKVIYHIDDE TTPYLVKIPI PSAQVTLRDF KLVLNKQNNN YKYFFKSMDA 

        70         80         90        100        110        120 
DFGVVKEEIA DDSTILPCFN GRVVSWLVSA DGTNQSDNCS ELPTSECELG MGLTNRKLQQ 

       130        140        150        160        170        180 
QQQQHQQQQQ QQQQQHQQQQ QQQQQQVQPV QLAQQQQQQV LHHQKMMGNP LLQPPPLTYQ 

       190        200        210        220        230        240 
SASVLSSDLD STSLFGTESE LTLDRDMTDY SSVQRLQVRK KPQRRKKRAP SMSRTSSYSS 

       250        260        270        280        290        300 
ITDSTMSLNI ITVSINMEAV NFLGISIVGQ SNRGGDGGIY VGSIMKGGAV ALDGRIEPGD 

       310        320        330        340        350        360 
MILQVNDVNF ENMTNDEAVR VLREVVQKPG PIKLVVAKCW DPNPKGYFTI PRTEPVRPID 

       370        380        390        400        410        420 
PGAWVAHTQA LTSHDSIIAD IAEPIKERLD QNNLEEIVKA MTKPDSGLEI RDRMWLKITI 

       430        440        450        460        470        480 
PNAFIGADAV NWVLENVEDV QDRREARRIV SAMLRSNYIK HTVNKLTFSE QCYYVVNEER 

       490        500        510        520        530        540 
NPNLLGRGHL HPHQLPHGHG GHALSHADTE SITSDIGPLP NPPIYMPYSA TYNPSHGYQP 

       550        560        570        580        590        600 
IQYGIAERHI SSGSSSSDVL TSKDISASQS DITSVIHQAN QLTIAAHGSN KSSGSSNRGG 

       610        620 
GGGGGGGGNN TNDQDVSVFN YVL 

References and documentation are available.

Number of amino acids: 623

Molecular weight: 68916.98

Theoretical pI: 5.89

Amino acid composition: 
Ala (A)  32	  5.1%
Arg (R)  29	  4.7%
Asn (N)  37	  5.9%
Asp (D)  39	  6.3%
Cys (C)   5	  0.8%
Gln (Q)  58	  9.3%
Glu (E)  28	  4.5%
Gly (G)  47	  7.5%
His (H)  19	  3.0%
Ile (I)  40	  6.4%
Leu (L)  44	  7.1%
Lys (K)  26	  4.2%
Met (M)  16	  2.6%
Phe (F)  12	  1.9%
Pro (P)  34	  5.5%
Ser (S)  57	  9.1%
Thr (T)  33	  5.3%
Trp (W)   5	  0.8%
Tyr (Y)  18	  2.9%
Val (V)  44	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 67
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      2985
Hydrogen    H	      4736
Nitrogen    N	       874
Oxygen      O	       961
Sulfur      S	        21

Formula: C2985H4736N874O961S21
Total number of atoms: 9577

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    54570
Abs 0.1% (=1 g/l)   0.792, assuming all pairs of Cys residues form cystines


Ext. coefficient    54320
Abs 0.1% (=1 g/l)   0.788, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.58
This classifies the protein as unstable.



Aliphatic index: 78.20

Grand average of hydropathicity (GRAVY): -0.583