ProtParam
User-provided sequence:
10 20 30 40 50 60
MDAIKKKMQA MKVDKDGALE RALVCEQEAR DANTRAEKAE EEARQLQKKI QTVENELDQT
70 80 90 100 110 120
QEALTLVTGK LEEKNKALQN AESEVAALNR RIQLLEEDLE RSEERLGSAT AKLSEASQAA
130 140 150 160 170 180
DESERARKIL ENRALADEER MDALENQLKE ARFLAEEADK KYDEVARKLA MVEADLERAE
190 200 210 220 230 240
ERAEQGENKI VELEEELRVV GNNLKSLEVS EEKANQREEE YKNQIKTLNT RLKEAEARAE
250 260 270 280 290 300
FAERSVQKLQ KEVDRLEDDL IVEKERYCMI GDSLDEAFVD LIKGLEPFWN PRNPKPPTPK
310 320 330 340 350 360
LPTPTPEELA AMEEARAAAE AAAAAEAEAA EAAAAAGEAG PDGAPAAPGE EKAPAKEPTP
370 380 390 400 410 420
PKEPTPPPPP PPPFEYSIDL PPEGAEVPYV KNYEPPPPGS EPEPVPAAEG EAAPAAEGAA
430 440 450 460 470 480
PPAEGAAPPA EGAVPPADGA APPAEGAAPA AEGAAPPADG AAPPAEAAAA PADAAAPAAE
490 500 510
AAPAEAPAAE ATAAEAPPAE AAPAEAAPAA AEGEAPPA
References and
documentation are available.
Number of amino acids: 518
Molecular weight: 54585.35
Theoretical pI: 4.39
Total number of negatively charged residues (Asp + Glu): 114
Total number of positively charged residues (Arg + Lys): 58
Atomic composition:
Carbon C 2355
Hydrogen H 3775
Nitrogen N 659
Oxygen O 811
Sulfur S 9
Formula: C
2355H
3775N
659O
811S
9
Total number of atoms: 7609
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 14565
Abs 0.1% (=1 g/l) 0.267, assuming all pairs of Cys residues form cystines
Ext. coefficient 14440
Abs 0.1% (=1 g/l) 0.265, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 52.11
This classifies the protein as unstable.
Aliphatic index: 68.63
Grand average of hydropathicity (GRAVY): -0.718