ProtParam
User-provided sequence:
10 20 30 40 50 60
MDAIKKKMQA MKVDKDGALE RALVCEQEAR DANTRAEKAE EEARQLQKKI QTVENELDQT
70 80 90 100 110 120
QEALTLVTGK LEEKNKALQN KKKTTKMTTS IPQGTLLDVL KKKMRQTKEE MEKYKDECEE
130 140 150 160 170 180
FHKRLQLEVV RREEAESEVA ALNRRIQLLE EDLERSEERL GSATAKLSEA SQAADESERA
190 200 210 220 230 240
RKILENRALA DEERMDALEN QLKEARFLAE EADKKYDEVA RKLAMVEADL ERAEERAEQG
250 260 270 280 290 300
ENKIVELEEE LRVVGNNLKS LEVSEEKANQ REEEYKNQIK TLNTRLKEAE ARAEFAERSV
310 320 330
QKLQKEVDRL EDDLVLEKER YKDIGDDLDT AFVELILKE
References and
documentation are available.
Number of amino acids: 339
Molecular weight: 39325.23
Theoretical pI: 4.92
Total number of negatively charged residues (Asp + Glu): 86
Total number of positively charged residues (Arg + Lys): 66
Atomic composition:
Carbon C 1674
Hydrogen H 2803
Nitrogen N 493
Oxygen O 573
Sulfur S 10
Formula: C
1674H
2803N
493O
573S
10
Total number of atoms: 5553
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 6085
Abs 0.1% (=1 g/l) 0.155, assuming all pairs of Cys residues form cystines
Ext. coefficient 5960
Abs 0.1% (=1 g/l) 0.152, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.91
This classifies the protein as unstable.
Aliphatic index: 81.53
Grand average of hydropathicity (GRAVY): -1.049