ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDAIKKKMQA MKVDKDGALE RALVCEQEAR DANTRAEKAE EEARQLQKKI QTVENELDQT 

        70         80         90        100        110        120 
QEALTLVTGK LEEKNKALQN KKKTTKMTTS IPQGTLLDVL KKKMRQTKEE MEKYKDECEE 

       130        140        150        160        170        180 
FHKRLQLEVV RREEAESEVA ALNRRIQLLE EDLERSEERL GSATAKLSEA SQAADESERA 

       190        200        210        220        230        240 
RKILENRALA DEERMDALEN QLKEARFLAE EADKKYDEVA RKLAMVEADL ERAEERAEQG 

       250        260        270        280        290        300 
ENKIVELEEE LRVVGNNLKS LEVSEEKANQ REEEYKNQIK TLNTRLKEAE ARAEFAERSV 

       310        320        330 
QKLQKEVDRL EDDLVLEKER YKDIGDDLDT AFVELILKE 

References and documentation are available.

Number of amino acids: 339

Molecular weight: 39325.23

Theoretical pI: 4.92

Amino acid composition: 
Ala (A)  37	 10.9%
Arg (R)  27	  8.0%
Asn (N)  13	  3.8%
Asp (D)  21	  6.2%
Cys (C)   2	  0.6%
Gln (Q)  19	  5.6%
Glu (E)  65	 19.2%
Gly (G)   7	  2.1%
His (H)   1	  0.3%
Ile (I)   9	  2.7%
Leu (L)  39	 11.5%
Lys (K)  39	 11.5%
Met (M)   8	  2.4%
Phe (F)   4	  1.2%
Pro (P)   1	  0.3%
Ser (S)  10	  2.9%
Thr (T)  15	  4.4%
Trp (W)   0	  0.0%
Tyr (Y)   4	  1.2%
Val (V)  18	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 86
Total number of positively charged residues (Arg + Lys): 66

Atomic composition:

Carbon      C	      1674
Hydrogen    H	      2803
Nitrogen    N	       493
Oxygen      O	       573
Sulfur      S	        10

Formula: C1674H2803N493O573S10
Total number of atoms: 5553

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     6085
Abs 0.1% (=1 g/l)   0.155, assuming all pairs of Cys residues form cystines


Ext. coefficient     5960
Abs 0.1% (=1 g/l)   0.152, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.91
This classifies the protein as unstable.



Aliphatic index: 81.53

Grand average of hydropathicity (GRAVY): -1.049