ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDCKILVLGA GGLGCEILKN LTMLSFVKQV HIVDIDTIEL TNLNRQFLFC DKDIGKPKAQ 

        70         80         90        100        110        120 
VAAQYVNTRF PQLEVVAHVQ DLTTLPPSFY KDFQFIISGL DAIEPRRFIN ETLVKLTLES 

       130        140        150        160        170        180 
NYEICIPFID GGTEGLKGHV KTIIPGITAC WECSIDTLPS QQDTVPMCTI ANNPRCIEHV 

       190        200        210        220        230        240 
VEYVSTIQYP DLNIESTADM EFLLEKCCER AAQFSISTEK LSTSFILGII KSIIPSVSTT 

       250        260        270        280        290 
NAMVAATCCT QMVKIYNDLI DLENGNNFTL INCSEGCFMY SFKFERLPDC TVCSNSNSN 

References and documentation are available.

Number of amino acids: 299

Molecular weight: 33278.43

Theoretical pI: 4.74

Amino acid composition: 
Ala (A)  14	  4.7%
Arg (R)   7	  2.3%
Asn (N)  18	  6.0%
Asp (D)  16	  5.4%
Cys (C)  16	  5.4%
Gln (Q)  12	  4.0%
Glu (E)  19	  6.4%
Gly (G)  14	  4.7%
His (H)   4	  1.3%
Ile (I)  30	 10.0%
Leu (L)  27	  9.0%
Lys (K)  15	  5.0%
Met (M)   7	  2.3%
Phe (F)  16	  5.4%
Pro (P)  13	  4.3%
Ser (S)  20	  6.7%
Thr (T)  24	  8.0%
Trp (W)   1	  0.3%
Tyr (Y)   7	  2.3%
Val (V)  19	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 22

Atomic composition:

Carbon      C	      1476
Hydrogen    H	      2340
Nitrogen    N	       374
Oxygen      O	       451
Sulfur      S	        23

Formula: C1476H2340N374O451S23
Total number of atoms: 4664

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    16930
Abs 0.1% (=1 g/l)   0.509, assuming all pairs of Cys residues form cystines


Ext. coefficient    15930
Abs 0.1% (=1 g/l)   0.479, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.58
This classifies the protein as stable.



Aliphatic index: 97.46

Grand average of hydropathicity (GRAVY): 0.138