ProtParam
User-provided sequence:
10 20 30 40 50 60
MDDDIAALVV DNGSGMCKAG FAGDDAPLAV FPSIVGRPRH QGVMVGMGQK DSYVGDEAQS
70 80 90 100 110 120
KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPL NPKANREKMT
130 140 150 160 170 180
QIMFETFNTP AMYVAIQAVL SLYASGRTTG IVMDSGDGVT HTVPIYEGYA LPHAILRLDL
190 200 210 220 230 240
AGRDLTDYLM KILTERGYSF TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY
250 260 270 280 290 300
ELPDGQVITI GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS
310 320 330 340 350 360
GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS TFQQMWISKQ
370
EYDESGPSIV HRKCF
References and
documentation are available.
Number of amino acids: 375
Molecular weight: 41693.71
Theoretical pI: 5.21
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 1850
Hydrogen H 2902
Nitrogen N 488
Oxygen O 561
Sulfur S 23
Formula: C
1850H
2902N
488O
561S
23
Total number of atoms: 5824
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 44725
Abs 0.1% (=1 g/l) 1.073, assuming all pairs of Cys residues form cystines
Ext. coefficient 44350
Abs 0.1% (=1 g/l) 1.064, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.50
This classifies the protein as stable.
Aliphatic index: 82.99
Grand average of hydropathicity (GRAVY): -0.178