ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDDVAGLQEA AGARFSQIEL IGRGSFGDVY KAFDKDLNKE VAIKVIDLEE SEDEIEDIQK 

        70         80         90        100        110        120 
EISVLSQCRC PYITEYYGSY LHQTKLWIIM EYMAGGSVAD LLQSNNPLDE TSIACITRDL 

       130        140        150        160        170        180 
LHAVEYLHNE GKIHRDIKAA NILLSENGDV KVADFGVSAQ LTRTISRRKT FVGTPFWMAP 

       190        200        210        220        230        240 
EVIQNSEGYN EKADIWSLGI TVIEMAKGEP PLADLHPMRV LFIIPRETPP QLDEHFSRQV 

       250        260        270        280        290        300 
KEFVSLCLKK APAERPSAKE LIKHRFIKNA RKSPKLLERI RERPKYQVKE DEETPRNGAK 

       310        320        330        340        350        360 
APVESSGTVR IARDERSQGA PGYSFQGNTV KNAGWDFTVG GSQSIGTVRA LKPPQARERR 

       370        380        390        400        410        420 
QEVSPNRISQ RTTRPSGNQW SSATGSTISE ASEGGFVRRH PFQNDHEDGF HEEDDSSLSG 

       430        440        450        460        470        480 
SGTVVIRTPR SSQSSSVFRE PSSGSSGRYA AFDDASASGT VVVRGQYDDS GSPRTPKSRL 

       490        500        510        520        530        540 
GIQERTSSAS EDSNANLAEA KAALDAGFRR GKARERLGMG NNNNDGKVNR RREQMADDSD 

       550        560        570        580        590        600 
YSRNSGDKSS KQKVVPRSEQ VSDEEDDSIW ESLPASLSVL LIPSLKEALG DDSKESTVRT 

       610        620        630        640        650        660 
VSRSLVMMER EKPGSCEAFV AKLIELLGSS KEASVKELHD MAVCVFAKTT PDNAENKMKQ 

       670        680        690 
ANKEFSSNTN VSPLGRFLLS RWLGQSSRDL 

References and documentation are available.

Number of amino acids: 690

Molecular weight: 76318.16

Theoretical pI: 6.03

Amino acid composition: 
Ala (A)  53	  7.7%
Arg (R)  53	  7.7%
Asn (N)  28	  4.1%
Asp (D)  43	  6.2%
Cys (C)   6	  0.9%
Gln (Q)  27	  3.9%
Glu (E)  59	  8.6%
Gly (G)  48	  7.0%
His (H)  11	  1.6%
Ile (I)  34	  4.9%
Leu (L)  48	  7.0%
Lys (K)  42	  6.1%
Met (M)  12	  1.7%
Phe (F)  22	  3.2%
Pro (P)  32	  4.6%
Ser (S)  80	 11.6%
Thr (T)  29	  4.2%
Trp (W)   7	  1.0%
Tyr (Y)  13	  1.9%
Val (V)  43	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 102
Total number of positively charged residues (Arg + Lys): 95

Atomic composition:

Carbon      C	      3298
Hydrogen    H	      5279
Nitrogen    N	       975
Oxygen      O	      1072
Sulfur      S	        18

Formula: C3298H5279N975O1072S18
Total number of atoms: 10642

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    58245
Abs 0.1% (=1 g/l)   0.763, assuming all pairs of Cys residues form cystines


Ext. coefficient    57870
Abs 0.1% (=1 g/l)   0.758, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.31
This classifies the protein as unstable.



Aliphatic index: 72.10

Grand average of hydropathicity (GRAVY): -0.658