ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDDYTREMMD LKTLVTRTLE KKGVLAKIRA ELRASVFEAI EEEDRVIENN EGPPPALLGS 

        70         80         90        100        110        120 
CNDRARKLHA SPSGRLLSAL ICEYLDWAQL NHTLIVYQPE SNLPKDSWKS ELRDFNSNNG 

       130        140        150        160        170        180 
FELNRNGDSG PLLLDVLEGF LKFESMTQGM GSSSRRDSET ESSSSLESRN PPRRSSASDS 

       190        200        210        220        230        240 
LPPQRRPVSA SQASDRRAGL STSGYRKDEF NWRQGNQDTH EEVTRASAAL ENLQLDRKTR 

       250        260 
NLTSSWRNVR DGTNEEEGRD 

References and documentation are available.

Number of amino acids: 260

Molecular weight: 29334.31

Theoretical pI: 5.36

Amino acid composition: 
Ala (A)  16	  6.2%
Arg (R)  27	 10.4%
Asn (N)  17	  6.5%
Asp (D)  18	  6.9%
Cys (C)   2	  0.8%
Gln (Q)   8	  3.1%
Glu (E)  25	  9.6%
Gly (G)  15	  5.8%
His (H)   3	  1.2%
Ile (I)   5	  1.9%
Leu (L)  29	 11.2%
Lys (K)  10	  3.8%
Met (M)   5	  1.9%
Phe (F)   6	  2.3%
Pro (P)  12	  4.6%
Ser (S)  32	 12.3%
Thr (T)  13	  5.0%
Trp (W)   4	  1.5%
Tyr (Y)   4	  1.5%
Val (V)   9	  3.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1245
Hydrogen    H	      1998
Nitrogen    N	       386
Oxygen      O	       421
Sulfur      S	         7

Formula: C1245H1998N386O421S7
Total number of atoms: 4057

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    28085
Abs 0.1% (=1 g/l)   0.957, assuming all pairs of Cys residues form cystines


Ext. coefficient    27960
Abs 0.1% (=1 g/l)   0.953, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.94
This classifies the protein as unstable.



Aliphatic index: 67.19

Grand average of hydropathicity (GRAVY): -0.947