ProtParam
User-provided sequence:
10 20 30 40 50 60
MDDYTREMMD LKTLVTRTLE KKGVLAKIRA ELRASVFEAI EEEDRVIENN EGPPPALLGS
70 80 90 100 110 120
CNDRARKLHA SPSGRLLSAL ICEYLDWAQL NHTLIVYQPE SNLPKDSWKS ELRDFNSNNG
130 140 150 160 170 180
FELNRNGDSG PLLLDVLEGF LKFESMTQGM GSSSRRDSET ESSSSLESRN PPRRSSASDS
190 200 210 220 230 240
LPPQRRPVSA SQASDRRAGL STSGYRKDEF NWRQGNQDTH EEVTRASAAL ENLQLDRKTR
250 260
NLTSSWRNVR DGTNEEEGRD
References and
documentation are available.
Number of amino acids: 260
Molecular weight: 29334.31
Theoretical pI: 5.36
Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 37
Atomic composition:
Carbon C 1245
Hydrogen H 1998
Nitrogen N 386
Oxygen O 421
Sulfur S 7
Formula: C
1245H
1998N
386O
421S
7
Total number of atoms: 4057
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 28085
Abs 0.1% (=1 g/l) 0.957, assuming all pairs of Cys residues form cystines
Ext. coefficient 27960
Abs 0.1% (=1 g/l) 0.953, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 53.94
This classifies the protein as unstable.
Aliphatic index: 67.19
Grand average of hydropathicity (GRAVY): -0.947