ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDEASHLPNG SLKNMEFTPV GFIKSKRNTT QTQVVSPTKV PNANNGDENE GPVKKRQRRS 

        70         80         90        100        110        120 
IDDTIDSTRL FSEASQFDDS FPEIKANIPP SPRSGNVDKS RKRNLIDDLK KDVPMSQPLK 

       130        140        150        160        170        180 
EQEVREHQMK KERFDRALES KLLGKRHITY ANSDISNKEL YINEIKSLKH EIKELRKEKN 

       190        200        210        220        230        240 
DTLNNYDTLE EETDDLKNRL QALEKELDAK NKIVNSRKVD DHSGCIEERE QMERKLAELE 

       250        260        270        280        290        300 
RKLKTVKDQV LELENNSDVQ SLKLRSKEDE LKNLMNELNE LKSNAEEKDT QLEFKKNELR 

       310        320        330        340        350        360 
KRTNELNELK IKSDEMDLQL KQKQNESKRL KDELNELETK FSENGSQSSA KENELKMLKN 

       370        380        390        400        410        420 
KIAELEEEIS TKNSQLIAKE GKLASLMAQL TQLESKLNQR DSQLGSREEE LKKTNDKLQK 

       430        440        450        460        470        480 
DIRIAREETV SKDERIIDLQ KKVKQLENDL FVIKKTHSES KTITDNELES KDKLIKILEN 

       490        500        510        520        530        540 
DLKVAQEKYS KMEKELKERE FNYKISESKL EDEKTTLNEK ISNLAAENSQ LKNKIEDNST 

       550        560        570        580        590        600 
ATHHMKENYE KQLESLRKDI EEYKESAKDS EDKIEELKIR IAENSAKVSE KRSKDIKQKD 

       610        620        630        640        650        660 
EQISDLTQNL KLQEDEISSL KSIIDRYKKD FNQLKSEQSN IQHDLNLQIL NLENKLIESE 

       670        680        690        700        710        720 
DELKSLRDSQ KIEIENWKRK YNNLSLENDR LLTEKESASD KEREISILNR KLDEMDKEKW 

       730        740        750        760        770        780 
NLQESKEKYK RELQKVITAN DRLRREKEEL NENSNNIRIM EDKMTRIKKN YLSEITSLQE 

       790        800        810        820        830        840 
ENRRLEERLI LNERRKDNDS TMQLNDIISY YKLKYHSEVR HNNDLKVIND YLNKVLALGT 

       850        860        870        880        890        900 
RRLRLDTRKG EHSLNISLPD DDELDRDYYN SHVYTRYHDY EYPLRFNLNR RGPYFERRLS 

       910        920        930        940 
FKTVALLVLA CVRMKRIAFY RRSDDNRLRI LRDRIESSSG RISW 

References and documentation are available.

Number of amino acids: 944

Molecular weight: 111781.76

Theoretical pI: 6.65

Amino acid composition: 
Ala (A)  29	  3.1%
Arg (R)  69	  7.3%
Asn (N)  78	  8.3%
Asp (D)  71	  7.5%
Cys (C)   2	  0.2%
Gln (Q)  40	  4.2%
Glu (E) 123	 13.0%
Gly (G)  14	  1.5%
His (H)  14	  1.5%
Ile (I)  58	  6.1%
Leu (L) 111	 11.8%
Lys (K) 122	 12.9%
Met (M)  16	  1.7%
Phe (F)  15	  1.6%
Pro (P)  14	  1.5%
Ser (S)  80	  8.5%
Thr (T)  36	  3.8%
Trp (W)   3	  0.3%
Tyr (Y)  23	  2.4%
Val (V)  26	  2.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 194
Total number of positively charged residues (Arg + Lys): 191

Atomic composition:

Carbon      C	      4815
Hydrogen    H	      7952
Nitrogen    N	      1422
Oxygen      O	      1590
Sulfur      S	        18

Formula: C4815H7952N1422O1590S18
Total number of atoms: 15797

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    50895
Abs 0.1% (=1 g/l)   0.455, assuming all pairs of Cys residues form cystines


Ext. coefficient    50770
Abs 0.1% (=1 g/l)   0.454, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.33
This classifies the protein as unstable.



Aliphatic index: 80.88

Grand average of hydropathicity (GRAVY): -1.220