ProtParam
User-provided sequence:
10 20 30 40 50 60
MDEGVIAVSA MDAFEKLEKV GEGTYGKVYR AREKATGKIV ALKKTRLHED EEGVPSTTLR
70 80 90 100 110 120
EISILRMLAR DPHVVRLMDV KQGLSKEGKT VLYLVFEYMD TDVKKFIRSF RSTGKNIPTQ
130 140 150 160 170 180
TIKSLMYQLC KGMAFCHGHG ILHRDLKPHN LLMDPKTMRL KIADLGLARA FTLPMKKYTH
190 200 210 220 230 240
EILTLWYRAP EVLLGATHYS TAVDMWSVGC IFAELVTNQA IFQGDSELQQ LLHIFKLFGT
250 260 270 280 290 300
PNEEMWPGVS TLKNWHEYPQ WKPSTLSSAV PNLDEAGVDL LSKMLQYEPA KRISAKMAME
310
HPYFDDLPEK SSL
References and
documentation are available.
Number of amino acids: 313
Molecular weight: 35593.45
Theoretical pI: 8.54
Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 41
Atomic composition:
Carbon C 1603
Hydrogen H 2540
Nitrogen N 424
Oxygen O 454
Sulfur S 18
Formula: C
1603H
2540N
424O
454S
18
Total number of atoms: 5039
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 44015
Abs 0.1% (=1 g/l) 1.237, assuming all pairs of Cys residues form cystines
Ext. coefficient 43890
Abs 0.1% (=1 g/l) 1.233, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.99
This classifies the protein as stable.
Aliphatic index: 87.86
Grand average of hydropathicity (GRAVY): -0.255