ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDEHTLNDLL ECSVCLERLD TTSKVLPCQH TFCRKCLQDI VASQHKLRCP ECRILVSCKI 

        70         80         90        100        110        120 
DELPPNVLLM RILEGMKQNA AAGKGEEKGE ETETQPERAK PQPPAESVAP PDNQLLQLQS 

       130        140        150        160        170        180 
HQQSHQPARH KQRRFLLPHA YALFDFASGE ATDLKFKKGD LILIKHRIDN NWFVGQANGQ 

       190        200        210        220        230        240 
EGTFPINYVK VSVPLPMPQC IAMYDFKMGP NDEEGCLEFK KSTVIQVMRR VDHNWAEGRI 

       250        260        270        280        290        300 
GQTIGIFPIA FVELNAAAKK LLDSGLHTHP FCHPPKQQGQ RALPPVPVID PTVVTESSSG 

       310        320        330        340        350        360 
SSNSTPGSSN SSSTSSSNNC SPNHQISLPN TPQHVVASGS ASVRFRDKGA KEKRHSLNAL 

       370        380        390        400        410        420 
LGGGAPLSLL QTNRHSAEIL SLPHELSRLE VSSSTALKPT SAPQTSRVLK TTVQQQMQPN 

       430        440        450        460        470        480 
LPWGYLALFP YKPRQTDELE LKKGCVYIVT ERCVDGWFKG KNWLDITGVF PGNYLTPLRA 

       490        500        510        520        530        540 
RDQQQLMHQW KYVPQNADAQ MAQVQQHPVA PDVRLNNMLS MQPPDLPPRQ QQATATTTSC 

       550        560        570        580        590        600 
SVWSKPVEAL FSRKSEPKPE TATASTTSSS SSGAVGLMRR LTHMKTRSKS PGASLQQVPK 

       610        620        630        640        650        660 
EAISTNVEFT TNPSAKLHPV HVRSGSCPSQ LQHSQPLNET PAAKTAAQQQ QFLPKQLPSA 

       670        680        690        700        710        720 
STNSVSYGSQ RVKGSKERPH LICARQSLDA ATFRSMYNNA ASPPPPTTSV APAVYAGGQQ 

       730        740        750        760        770        780 
QVIPGGGAQS QLHANMIIAP SHRKSHSLDA SHVLSPSSNM ITEAAIKASA TTKSPYCTRE 

       790        800        810        820        830 
SRFRCIVPYP PNSDIELELH LGDIIYVQRK QKNGWYKGTH ARTHKTGLFP ASFVEPDC 

References and documentation are available.

Number of amino acids: 838

Molecular weight: 92057.56

Theoretical pI: 9.20

Amino acid composition: 
Ala (A)  66	  7.9%
Arg (R)  41	  4.9%
Asn (N)  35	  4.2%
Asp (D)  28	  3.3%
Cys (C)  20	  2.4%
Gln (Q)  62	  7.4%
Glu (E)  40	  4.8%
Gly (G)  44	  5.3%
His (H)  31	  3.7%
Ile (I)  30	  3.6%
Leu (L)  72	  8.6%
Lys (K)  49	  5.8%
Met (M)  17	  2.0%
Phe (F)  23	  2.7%
Pro (P)  73	  8.7%
Ser (S)  84	 10.0%
Thr (T)  52	  6.2%
Trp (W)   8	  1.0%
Tyr (Y)  15	  1.8%
Val (V)  48	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 90

Atomic composition:

Carbon      C	      4026
Hydrogen    H	      6411
Nitrogen    N	      1177
Oxygen      O	      1223
Sulfur      S	        37

Formula: C4026H6411N1177O1223S37
Total number of atoms: 12874

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    67600
Abs 0.1% (=1 g/l)   0.734, assuming all pairs of Cys residues form cystines


Ext. coefficient    66350
Abs 0.1% (=1 g/l)   0.721, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 53.73
This classifies the protein as unstable.



Aliphatic index: 71.96

Grand average of hydropathicity (GRAVY): -0.527