ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDELLGEALS AENQTGESTV ESEKLVTPED VMTISSLEQR TLNPDLFLYK ELVKAHLGER 

        70         80         90        100        110        120 
AASVIGMLVA LGRLSVRELV EKIDGMDVDS VKTTLVSLTQ LRCVKYLQET AISGKKTTYY 

       130        140        150        160        170        180 
YYNEEGIHIL LYSGLIIDEI ITQMRVNDEE EHKQLVAEIV QNVISLGSLT VEDYLSSVTS 

       190        200        210        220        230        240 
DSMKYTISSL FVQLCEMGYL IQISKLHYTP IEDLWQFLYE KHYKNIPRNS PLSDLKKRSQ 

       250        260        270        280        290        300 
AKMNAKTDFA KIINKPNELS QILTVDPKTS LRIVKPTVSL TINLDRFMKG RRSKQLINLA 

       310        320        330        340        350        360 
KTRVGSVTAQ VYKIALRLTE QKSPKIRDPL TQTGLLQDLE EAKSFQDEAE LVEEKTPGLT 

       370        380        390        400        410        420 
FNAIDLARHL PAELDLRGSL LSRKPSDNKK RSGSNAAASL PSKKLKTEDG FVIPALPAAV 

       430        440        450        460        470        480 
SKSLQESGDT QEEDEEEEDL DADTEDPHSA SLINSHLKIL ASSNFPFLNE TKPGVYYVPY 

       490        500        510        520        530        540 
SKLMPVLKSS VYEYVIASTL GPSAMRLSRC IRDNKLVSEK IINSTALMKE KDIRSTLASL 

       550        560        570        580        590        600 
IRYNSVEIQE VPRTADRSAS RAVFLFRCKE THSYNFMRQN LEWNMANLLF KKEKLKQENS 

       610        620        630        640        650 
TLLKKANRDD VKGRENELLL PSELNQLKMV NERELNVFAR LSRLLSLWEV FQMA 

References and documentation are available.

Number of amino acids: 654

Molecular weight: 74016.93

Theoretical pI: 6.26

Amino acid composition: 
Ala (A)  39	  6.0%
Arg (R)  34	  5.2%
Asn (N)  31	  4.7%
Asp (D)  33	  5.0%
Cys (C)   4	  0.6%
Gln (Q)  25	  3.8%
Glu (E)  58	  8.9%
Gly (G)  22	  3.4%
His (H)   9	  1.4%
Ile (I)  36	  5.5%
Leu (L)  88	 13.5%
Lys (K)  53	  8.1%
Met (M)  16	  2.4%
Phe (F)  16	  2.4%
Pro (P)  24	  3.7%
Ser (S)  63	  9.6%
Thr (T)  38	  5.8%
Trp (W)   3	  0.5%
Tyr (Y)  21	  3.2%
Val (V)  41	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 91
Total number of positively charged residues (Arg + Lys): 87

Atomic composition:

Carbon      C	      3276
Hydrogen    H	      5336
Nitrogen    N	       886
Oxygen      O	      1015
Sulfur      S	        20

Formula: C3276H5336N886O1015S20
Total number of atoms: 10533

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    48040
Abs 0.1% (=1 g/l)   0.649, assuming all pairs of Cys residues form cystines


Ext. coefficient    47790
Abs 0.1% (=1 g/l)   0.646, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.56
This classifies the protein as unstable.



Aliphatic index: 98.09

Grand average of hydropathicity (GRAVY): -0.357