ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDENEFDNQR ENKAVARVII SFLKYEEYAL KEIYNLRVKK WASISDRQKD MVPNYTKYLA 

        70         80         90        100        110        120 
NLKAAIIENG KFFRSVAEYA LQSISFEPGE IVQPNDLDMS KTCSLLTQVY REWSAEAISE 

       130        140        150        160        170        180 
RNCLNSRLVP FLKTLSPPKA DILIPGCGTG RLLVDLSRMG YNCEGNEFSY HMLLVSQYML 

       190        200        210        220        230        240 
NAGLLQNQII IYPFIHCFSH WKKIEDQLSP IKVPDIEAWS SNKGMGSMSI CAGSFVDCYG 

       250        260        270        280        290        300 
RNQGTKISSH YTFSRRMQLS RAKAENSKDV VVTNFFIDTG SNILDYLDTI GHVLKPGGIW 

       310        320        330        340        350        360 
CNFGPLLYHF ENDHGVETTY EVNPYSGFQD KINDYTPLMG LELSSDDIIS IATNHLDFEL 

       370        380        390        400 
IRRESGILCG YGRYAGPESC AMPGYMCHYW ILKSNPTNES 

References and documentation are available.

Number of amino acids: 400

Molecular weight: 45496.79

Theoretical pI: 5.67

Amino acid composition: 
Ala (A)  20	  5.0%
Arg (R)  17	  4.2%
Asn (N)  26	  6.5%
Asp (D)  21	  5.2%
Cys (C)  11	  2.8%
Gln (Q)  12	  3.0%
Glu (E)  26	  6.5%
Gly (G)  26	  6.5%
His (H)   9	  2.2%
Ile (I)  31	  7.8%
Leu (L)  36	  9.0%
Lys (K)  22	  5.5%
Met (M)  12	  3.0%
Phe (F)  17	  4.2%
Pro (P)  17	  4.2%
Ser (S)  36	  9.0%
Thr (T)  15	  3.8%
Trp (W)   6	  1.5%
Tyr (Y)  22	  5.5%
Val (V)  18	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 39

Atomic composition:

Carbon      C	      2033
Hydrogen    H	      3127
Nitrogen    N	       535
Oxygen      O	       606
Sulfur      S	        23

Formula: C2033H3127N535O606S23
Total number of atoms: 6324

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    66405
Abs 0.1% (=1 g/l)   1.460, assuming all pairs of Cys residues form cystines


Ext. coefficient    65780
Abs 0.1% (=1 g/l)   1.446, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.47
This classifies the protein as stable.



Aliphatic index: 83.38

Grand average of hydropathicity (GRAVY): -0.284