ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDFDNAKENI QPLASGRNVS LLQASLSQDS THGQELLAQR KQMEEEVRTY KGADPLGAWY 

        70         80         90        100        110        120 
TFICWIEQSY PAGGSGSGLQ TVLHQCLTKF EDDERYRQDK RLIKLFIKFM EKQKDKIEFY 

       130        140        150        160        170        180 
QQMYNNGIGT MLADFYIAWA YSYDLSGNMR KADEIFRLGL ECRAEPLEDL KEAHHHFGYT 

       190        200        210        220        230        240 
VGQRMLYSTG EEANAVNQEL NERRLALQSL HGRRQQISNS ITVGSIRTGA AVKSGLPGVV 

       250        260        270        280        290        300 
QVEPSTTTSR RNVGRNVEVF NDENSEGNIP ISNSETEVKS SLRSIIDSAC SQENIKEPVA 

       310        320        330        340        350        360 
WNKANAKRHK HGKIFGSNAS PDLGFDIHVD EQAMPPITNY ERRLEQPFRF PTNFVAKNRP 

       370        380        390        400        410        420 
QEPWVTPVTI EDEPNASGLP CYNKCLLYPR PNIEFSPEEY RAYSFLKHRN PQHPFIHRND 

       430        440        450        460        470        480 
DWWGTGRVIR GIRCYPNFAR YSKPQDLDEL DKFWKPPLVP GLQVVFDKIY NEVEQKEYQP 

       490        500        510        520        530        540 
EELLAAKWME KRNVTVHGDF DMEETVCLPG NKMPRRKSFF PSSFRKSIMP RRVSSVQEED 

       550        560        570        580        590        600 
EKKEDEATLI VKDIPSQVAP KIPESPCDQI DEPKIKIFED SGSPRDNFAV PAVPVPKIEI 

       610        620        630        640        650        660 
YEDSEEPQPT PKTRPFYDGD ETCSTQMFNM FIKSQAVSTP KGTQKQAPSR QFGTVLKELP 

       670        680        690        700        710        720 
LPEEAAHSVE SPVETRSPTL RKQLSTILET SEHGTQSLAT SGTTTKSTIT STSSPGSNAV 

       730        740        750        760        770        780 
SKLGSKIEEN TPGQMRLQRV ISAGVSAVLS EPEKPGGDNF LRRELWEPNA PSVPMLKSLR 

       790        800        810        820        830        840 
FQEDKTETIP RPLACFQEDK TETLPQMPIA LPENSMDHGS LGAENCFSAP QLPTLEDESD 

       850        860        870        880        890        900 
LCGLFGKTPP KTNIFGSPKR NFDPTSQLFK STQGDRRSFA VTGNKLEAAP SISNLQDSFM 

       910        920        930        940        950        960 
CDLSFVPETQ PEAVAKSGAI QKFDIFLDDT QPKLTKSKPV ALMVSSFDLD CTLPETQQVA 

       970        980        990       1000       1010       1020 
IKEPGTQQEN IGHSHMIASF MKDCTEIGSC PSQMPASVVK TNISSTSNKI KFSNDSMSES 

      1030       1040       1050       1060       1070       1080 
SRKAPVNENV GISKQPDEFF ELNAATEMFA TNISMIKNST LLIPNAKVAE ASELSIYYKT 

      1090       1100       1110       1120       1130       1140 
TPLTPKQSHR SWSQSDLETP PRENFVHPTS NGDQTVLNET LADANKNPFN VELISSLLES 

      1150       1160       1170       1180       1190       1200 
IDFSMYIEKL PHCQLVGHVK RLHPNTHLEV HNEKFEVSKM IGKGAYGSVY VGKHLKSGKK 

      1210       1220       1230       1240       1250       1260 
VALKQERPTN YWEFYICLEI HSRLTSEQMI PSYAHIDYAL VGNNSSVYIS EFSDYGSLIG 

      1270       1280       1290       1300       1310       1320 
VCNKVKSVTN RNMDEYVVMH LSCQMLDIVD HLHAMGIIHA DIKPDNFLLM KPICADPNEV 

      1330       1340       1350       1360       1370       1380 
SLQLIDFGVS IDMKLFPDNQ TFNYVHHDDL FKCIEMRTGR PWTYQLDLYG LVSVMHVLLF 

      1390       1400       1410       1420       1430       1440 
GRYMEVVQRS PSTIWMPKTN VPRYFQRTMW ENIFRTLLNI RDCRTMPNLQ QLRTQLKCAL 

      1450       1460 
AEKEKYVAEA ISKFNTILQK 

References and documentation are available.

Number of amino acids: 1460

Molecular weight: 165060.93

Theoretical pI: 5.93

Amino acid composition: 
Ala (A)  77	  5.3%
Arg (R)  69	  4.7%
Asn (N)  77	  5.3%
Asp (D)  73	  5.0%
Cys (C)  25	  1.7%
Gln (Q)  73	  5.0%
Glu (E) 115	  7.9%
Gly (G)  71	  4.9%
His (H)  35	  2.4%
Ile (I)  80	  5.5%
Leu (L) 112	  7.7%
Lys (K)  96	  6.6%
Met (M)  41	  2.8%
Phe (F)  65	  4.5%
Pro (P) 101	  6.9%
Ser (S) 128	  8.8%
Thr (T)  85	  5.8%
Trp (W)  15	  1.0%
Tyr (Y)  40	  2.7%
Val (V)  82	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 188
Total number of positively charged residues (Arg + Lys): 165

Atomic composition:

Carbon      C	      7294
Hydrogen    H	     11422
Nitrogen    N	      1998
Oxygen      O	      2240
Sulfur      S	        66

Formula: C7294H11422N1998O2240S66
Total number of atoms: 23020

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   143600
Abs 0.1% (=1 g/l)   0.870, assuming all pairs of Cys residues form cystines


Ext. coefficient   142100
Abs 0.1% (=1 g/l)   0.861, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 55.02
This classifies the protein as unstable.



Aliphatic index: 72.85

Grand average of hydropathicity (GRAVY): -0.552