ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDFLNDDELD LDLPVTAEIS KELFATEIEK YRESETNGTD VDNFDVDRFL VQKNFHYLPL 

        70         80         90        100        110        120 
DSLIRDLSGL SQKMVQTLLE QIRSNYDDYL TFSNTYTDEE NETLINLEKT QSDLQKFMTQ 

       130        140        150        160        170        180 
LDHLIKDDIS NTQEIIKDVL EYLKKLDEIY GSLRNHSQLT EALSLGKRLS KSLHEMCGIE 

       190        200        210        220        230        240 
PLEEEICSGL IEQLYKLITA SRRILESCAD SNSPYIHHLR NDYQDLLQEF QISLKILTEK 

       250        260 
CLENPSSLQN LSLTLVSIIK TA 

References and documentation are available.

Number of amino acids: 262

Molecular weight: 30325.16

Theoretical pI: 4.51

Amino acid composition: 
Ala (A)   6	  2.3%
Arg (R)   9	  3.4%
Asn (N)  14	  5.3%
Asp (D)  23	  8.8%
Cys (C)   4	  1.5%
Gln (Q)  14	  5.3%
Glu (E)  26	  9.9%
Gly (G)   6	  2.3%
His (H)   6	  2.3%
Ile (I)  20	  7.6%
Leu (L)  43	 16.4%
Lys (K)  16	  6.1%
Met (M)   4	  1.5%
Phe (F)   8	  3.1%
Pro (P)   5	  1.9%
Ser (S)  24	  9.2%
Thr (T)  17	  6.5%
Trp (W)   0	  0.0%
Tyr (Y)  10	  3.8%
Val (V)   7	  2.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 25

Atomic composition:

Carbon      C	      1336
Hydrogen    H	      2133
Nitrogen    N	       345
Oxygen      O	       440
Sulfur      S	         8

Formula: C1336H2133N345O440S8
Total number of atoms: 4262

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    15150
Abs 0.1% (=1 g/l)   0.500, assuming all pairs of Cys residues form cystines


Ext. coefficient    14900
Abs 0.1% (=1 g/l)   0.491, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.61
This classifies the protein as unstable.



Aliphatic index: 103.82

Grand average of hydropathicity (GRAVY): -0.429